Separation and dual detection of prostate cancer cells and protein biomarkers using a microchip device

Wanfeng Huang, Chun Li Chang, Norman D. Brault, Onur Gur, Zhe Wang, Shadia Jalal, Philip S. Low, Timothy L. Ratliff, Roberto Pili, Cagri A. Savran

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Current efforts for the detection of prostate cancer using only prostate specific antigen are not ideal and indicate a need to develop new assays-using multiple targets-that can more accurately stratify disease states. We previously introduced a device capable of the concurrent detection of cellular and molecular markers from a single sample fluid. Here, an improved design, which achieves affinity as well as size-based separation of captured targets using antibody-conjugated magnetic beads and a silicon chip containing micro-apertures, is presented. Upon injection of the sample, the integration of magnetic attraction with the micro-aperture chip permits larger cell-bead complexes to be isolated in an upper chamber with the smaller protein-bead complexes and remaining beads passing through the micro-apertures into the lower chamber. This enhances captured cell purity for on chip quantification, allows the separate retrieval of captured cells and proteins for downstream analysis, and enables higher bead concentrations for improved multiplexed ligand targeting. Using LNCaP cells and prostate specific membrane antigen (PSMA) to model prostate cancer, the device was able to detect 34 pM of spiked PSMA and achieve a cell capture efficiency of 93% from culture media. LNCaP cells and PSMA were then spiked into diluted healthy human blood to mimic a cancer patient. The device enabled the detection of spiked PSMA (relative to endogenous PSMA) while recovering 85-90% of LNCaP cells which illustrated the potential of new assays for the diagnosis of prostate cancer.

Original languageEnglish (US)
Pages (from-to)415-428
Number of pages14
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume17
Issue number3
DOIs
StatePublished - 2017

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Biomarkers
Antigens
Prostatic Neoplasms
Cells
Proteins
Equipment and Supplies
Membranes
Assays
Silicon
Prostate-Specific Antigen
Culture Media
Antibodies
Blood
human glutamate carboxypeptidase II
Ligands
Fluids
Injections
Neoplasms

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biochemistry
  • Biomedical Engineering

Cite this

Separation and dual detection of prostate cancer cells and protein biomarkers using a microchip device. / Huang, Wanfeng; Chang, Chun Li; Brault, Norman D.; Gur, Onur; Wang, Zhe; Jalal, Shadia; Low, Philip S.; Ratliff, Timothy L.; Pili, Roberto; Savran, Cagri A.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 17, No. 3, 2017, p. 415-428.

Research output: Contribution to journalArticle

Huang, Wanfeng ; Chang, Chun Li ; Brault, Norman D. ; Gur, Onur ; Wang, Zhe ; Jalal, Shadia ; Low, Philip S. ; Ratliff, Timothy L. ; Pili, Roberto ; Savran, Cagri A. / Separation and dual detection of prostate cancer cells and protein biomarkers using a microchip device. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2017 ; Vol. 17, No. 3. pp. 415-428.
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