Sequence analysis of measles virus nucleocapsid transcripts in patients with Paget's disease

W. E. Friedrichs, S. V. Reddy, J. M. Bruder, T. Cundy, J. Cornish, F. R. Singer, G. David Roodman

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

It has been debated for almost 30 years whether Paget's disease of bone results from paramyxoviral infection of osteoclasts (OCs). Paramyxoviral-like nuclear inclusions are found in OCs from patients with Paget's disease, and measles virus (MV) or canine distemper virus (CDV) messenger RNA (mRNA) transcripts have been detected by in situ hybridization in bone cells from pagetic lesions. Furthermore, immunocytochemical studies have shown the presence of several paramyxoviral species in OCs from patients with Paget's disease. However, others have been unable to detect paramyxoviral transcripts in bone samples from patients with Paget's disease or marrow cultures from involved sites of patients with Paget's disease. Furthermore, no one has been able to isolate an infectious virus from pagetic bone samples or marrow cells from patients with Paget's disease, and a full-length viral gene has not been sequenced from pagetic samples. In this study, we have obtained the full-length sequence for the MV nucleocapsid (MVNP) gene in bone marrow from an involved site from a patient with Paget's disease and more than 700 base pairs (bps) of MVNP sequence in 3 other patients with Paget's disease. These sequences were undetectable in four normal marrow samples studied simultaneously. The sequences from the patients contained multiple mutations that differed from the Edmonston strain MVNP gene. These findings are consistent with the presence of a chronic MV infection in affected sites from these patients with Paget's disease.

Original languageEnglish (US)
Pages (from-to)145-151
Number of pages7
JournalJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume17
Issue number1
StatePublished - 2002
Externally publishedYes

Fingerprint

Nucleocapsid
Measles virus
Sequence Analysis
Osteoclasts
Bone Marrow
Bone and Bones
Canine Distemper Virus
Osteitis Deformans
Intranuclear Inclusion Bodies
Viral Genes
Virus Diseases
Base Pairing
Genes
In Situ Hybridization
Viruses
Messenger RNA
Mutation

Keywords

  • Measles virus nucleocapsid transcripts
  • Osteoclasts
  • Paget's disease
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Surgery

Cite this

Sequence analysis of measles virus nucleocapsid transcripts in patients with Paget's disease. / Friedrichs, W. E.; Reddy, S. V.; Bruder, J. M.; Cundy, T.; Cornish, J.; Singer, F. R.; Roodman, G. David.

In: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 17, No. 1, 2002, p. 145-151.

Research output: Contribution to journalArticle

@article{9b70a5d638804bc0a759a48a2921260a,
title = "Sequence analysis of measles virus nucleocapsid transcripts in patients with Paget's disease",
abstract = "It has been debated for almost 30 years whether Paget's disease of bone results from paramyxoviral infection of osteoclasts (OCs). Paramyxoviral-like nuclear inclusions are found in OCs from patients with Paget's disease, and measles virus (MV) or canine distemper virus (CDV) messenger RNA (mRNA) transcripts have been detected by in situ hybridization in bone cells from pagetic lesions. Furthermore, immunocytochemical studies have shown the presence of several paramyxoviral species in OCs from patients with Paget's disease. However, others have been unable to detect paramyxoviral transcripts in bone samples from patients with Paget's disease or marrow cultures from involved sites of patients with Paget's disease. Furthermore, no one has been able to isolate an infectious virus from pagetic bone samples or marrow cells from patients with Paget's disease, and a full-length viral gene has not been sequenced from pagetic samples. In this study, we have obtained the full-length sequence for the MV nucleocapsid (MVNP) gene in bone marrow from an involved site from a patient with Paget's disease and more than 700 base pairs (bps) of MVNP sequence in 3 other patients with Paget's disease. These sequences were undetectable in four normal marrow samples studied simultaneously. The sequences from the patients contained multiple mutations that differed from the Edmonston strain MVNP gene. These findings are consistent with the presence of a chronic MV infection in affected sites from these patients with Paget's disease.",
keywords = "Measles virus nucleocapsid transcripts, Osteoclasts, Paget's disease, Polymerase chain reaction",
author = "Friedrichs, {W. E.} and Reddy, {S. V.} and Bruder, {J. M.} and T. Cundy and J. Cornish and Singer, {F. R.} and Roodman, {G. David}",
year = "2002",
language = "English (US)",
volume = "17",
pages = "145--151",
journal = "Journal of Bone and Mineral Research",
issn = "0884-0431",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Sequence analysis of measles virus nucleocapsid transcripts in patients with Paget's disease

AU - Friedrichs, W. E.

AU - Reddy, S. V.

AU - Bruder, J. M.

AU - Cundy, T.

AU - Cornish, J.

AU - Singer, F. R.

AU - Roodman, G. David

PY - 2002

Y1 - 2002

N2 - It has been debated for almost 30 years whether Paget's disease of bone results from paramyxoviral infection of osteoclasts (OCs). Paramyxoviral-like nuclear inclusions are found in OCs from patients with Paget's disease, and measles virus (MV) or canine distemper virus (CDV) messenger RNA (mRNA) transcripts have been detected by in situ hybridization in bone cells from pagetic lesions. Furthermore, immunocytochemical studies have shown the presence of several paramyxoviral species in OCs from patients with Paget's disease. However, others have been unable to detect paramyxoviral transcripts in bone samples from patients with Paget's disease or marrow cultures from involved sites of patients with Paget's disease. Furthermore, no one has been able to isolate an infectious virus from pagetic bone samples or marrow cells from patients with Paget's disease, and a full-length viral gene has not been sequenced from pagetic samples. In this study, we have obtained the full-length sequence for the MV nucleocapsid (MVNP) gene in bone marrow from an involved site from a patient with Paget's disease and more than 700 base pairs (bps) of MVNP sequence in 3 other patients with Paget's disease. These sequences were undetectable in four normal marrow samples studied simultaneously. The sequences from the patients contained multiple mutations that differed from the Edmonston strain MVNP gene. These findings are consistent with the presence of a chronic MV infection in affected sites from these patients with Paget's disease.

AB - It has been debated for almost 30 years whether Paget's disease of bone results from paramyxoviral infection of osteoclasts (OCs). Paramyxoviral-like nuclear inclusions are found in OCs from patients with Paget's disease, and measles virus (MV) or canine distemper virus (CDV) messenger RNA (mRNA) transcripts have been detected by in situ hybridization in bone cells from pagetic lesions. Furthermore, immunocytochemical studies have shown the presence of several paramyxoviral species in OCs from patients with Paget's disease. However, others have been unable to detect paramyxoviral transcripts in bone samples from patients with Paget's disease or marrow cultures from involved sites of patients with Paget's disease. Furthermore, no one has been able to isolate an infectious virus from pagetic bone samples or marrow cells from patients with Paget's disease, and a full-length viral gene has not been sequenced from pagetic samples. In this study, we have obtained the full-length sequence for the MV nucleocapsid (MVNP) gene in bone marrow from an involved site from a patient with Paget's disease and more than 700 base pairs (bps) of MVNP sequence in 3 other patients with Paget's disease. These sequences were undetectable in four normal marrow samples studied simultaneously. The sequences from the patients contained multiple mutations that differed from the Edmonston strain MVNP gene. These findings are consistent with the presence of a chronic MV infection in affected sites from these patients with Paget's disease.

KW - Measles virus nucleocapsid transcripts

KW - Osteoclasts

KW - Paget's disease

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0036133370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036133370&partnerID=8YFLogxK

M3 - Article

C2 - 11771661

AN - SCOPUS:0036133370

VL - 17

SP - 145

EP - 151

JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

IS - 1

ER -