Sequence conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae

Kathryn J. Hofmann, Michael P. Neeper, Henry Z. Markus, Darron Brown, Martin Müller, Kathrin U. Jansen

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The major capsid protein L1 of human papillomaviruses (HPVs) has been identified as a promising candidate antigen for a prophylactic HPV vaccine. Since amino acid sequence heterogeneity has been demonstrated for the L1 genes within individual HPV types, nucleotide sequences for L1 were determined from six HPV-18 clinical isolates and the cervical carcinoma cell line SW756 and compared to the published HPV-18 prototype sequence. The sequences were almost identical between the clinical isolates and SW756 but differed markedly from the published prototype sequence. Resequencing the prototype HPV-18 revealed that these differences were due to sequencing artifacts of the prototype HPV-18 sequence archived in GenBank. Thus, the HPV-18 L1 genes seem to display a very high level of sequence conservation. The HPV-18 L1 gene derived from SW756 was expressed in Saccharomyces cerevisiae and self-assembly of the L1 protein into virus-like particles was demonstrated.

Original languageEnglish
Pages (from-to)465-468
Number of pages4
JournalJournal of General Virology
Volume77
Issue number3
StatePublished - 1996

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Papillomaviridae
Human papillomavirus 18
Capsid Proteins
Virion
Saccharomyces cerevisiae
Genes
Papillomavirus Vaccines
Nucleic Acid Databases
Artifacts
Amino Acid Sequence
Carcinoma
Antigens
Cell Line

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Sequence conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae. / Hofmann, Kathryn J.; Neeper, Michael P.; Markus, Henry Z.; Brown, Darron; Müller, Martin; Jansen, Kathrin U.

In: Journal of General Virology, Vol. 77, No. 3, 1996, p. 465-468.

Research output: Contribution to journalArticle

Hofmann, Kathryn J. ; Neeper, Michael P. ; Markus, Henry Z. ; Brown, Darron ; Müller, Martin ; Jansen, Kathrin U. / Sequence conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae. In: Journal of General Virology. 1996 ; Vol. 77, No. 3. pp. 465-468.
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