Sequence of the notch locus of Drosophila melanogaster: relationship of the encoded protein to mammalian clotting and growth factors.

S. Kidd, Mark Kelley, M. W. Young

Research output: Contribution to journalArticle

272 Citations (Scopus)

Abstract

The Notch locus is essential for proper differentiation of the ectoderm in Drosophila melanogaster. Notch corresponds to a 37-kilobase transcription unit that codes for a major 10.4-kilobase polyadenylated RNA. The DNA sequence of this transcription unit is presented, except for portions of the two largest intervening sequences. DNA sequences also were obtained from three Notch cDNA clones, allowing the 5' and 3' ends of the gene to be mapped, and the structures and locations of nine RNA coding regions to be determined. The major Notch transcript encodes a protein of 2,703 amino acids. The protein is probably associated with cell surfaces and carries an extracellular domain composed of 36 cysteine-rich repeating units, each of about 38 amino acids. The gene appears to have evolved by repeated tandem duplications of the DNA coding for the 38-amino-acid-long protein segments, followed by insertion of intervening sequences. These repeating protein segments are quite homologous to portions of mammalian clotting factors IX and X and to the product of the Caenorhabditis elegans developmental gene lin-12. They are also similar to mammalian growth hormones, typified by epidermal growth factor.

Original languageEnglish
Pages (from-to)3094-3108
Number of pages15
JournalMolecular and Cellular Biology
Volume6
Issue number9
StatePublished - Sep 1986
Externally publishedYes

Fingerprint

Blood Coagulation Factors
Drosophila melanogaster
Intercellular Signaling Peptides and Proteins
Amino Acids
Introns
Proteins
Developmental Genes
Factor X
Ectoderm
Factor IX
Caenorhabditis elegans
Epidermal Growth Factor
Growth Hormone
Genes
Cysteine
Complementary DNA
Clone Cells
RNA
Messenger RNA
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Sequence of the notch locus of Drosophila melanogaster : relationship of the encoded protein to mammalian clotting and growth factors. / Kidd, S.; Kelley, Mark; Young, M. W.

In: Molecular and Cellular Biology, Vol. 6, No. 9, 09.1986, p. 3094-3108.

Research output: Contribution to journalArticle

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