Sequence similarity of calreticulin with a Ca2+-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells

K. H. Krause, H. K B Simmerman, Larry Jones, K. P. Campbell

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

HL-60 cells possess a 60 kDa Ca2+-binding protein that is contained in a discrete subcellular compartment, referred to as calciosomes. Subcellular fractionation studies have suggested that, in HL-60 cells, this intracellular compartment is an Ins(1,4,5)P3-sensitive Ca2+ store. In order to investigate the structural relationship of the 60 kDa Ca2+-binding protein of HL-60 cells to other Ca2+-binding proteins, we have purified the protein by ammonium sulphate extraction, acid precipitation, and DEAE-cellulose and phenyl-Sepharose column chromatography. The N-terminal sequence of the protein shows 93% identity with rabbit muscle calreticulin, a recently cloned sarcoplasmic reticulum Ca2+-binding protein. No amino acid sequence similarity with calsequestrin was found, although the purified protein cross-reacted with anti-calsequestrin antibodies. The calreticulin-related protein of HL-60 cells might play a role as an intravesicular Ca2+-binding protein of an Ins(1,4,5)P3-sensitive Ca2+ store.

Original languageEnglish (US)
Pages (from-to)545-548
Number of pages4
JournalBiochemical Journal
Volume270
Issue number2
StatePublished - 1990
Externally publishedYes

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Calreticulin
HL-60 Cells
Carrier Proteins
Calsequestrin
Proteins
DEAE-Cellulose
Agarose Chromatography
Ammonium Sulfate
Sarcoplasmic Reticulum
Column chromatography
Anti-Idiotypic Antibodies
Amino Acid Sequence
Fractionation
Muscle
Rabbits
Muscles
Acids
Amino Acids
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sequence similarity of calreticulin with a Ca2+-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells. / Krause, K. H.; Simmerman, H. K B; Jones, Larry; Campbell, K. P.

In: Biochemical Journal, Vol. 270, No. 2, 1990, p. 545-548.

Research output: Contribution to journalArticle

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