Seroconversion of type B to O erythrocytes using recombinant Glycine max α-D-galactosidase

Nancy C. Vosnidou, Scott A. Johnson, Moon Moon Mitra, Donald C. Wells, Chun Qin Li, Maria L. Evans, Michael A. Harmata, John C. Walker, Daniel Smith

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recombinant α-D-galactosidase (rGal) from soybean (Glycine max) hydrolyzed the immunodominant α-D-galactose residue from the B epitope of red blood cells. This converted type B erythrocytes to type O which are 'universally' transfusable. Type B red blood cells were obtained from four different donors and enzymatically converted. Cell function parameters, including red cell indices, pH, methemoglobin, carboxyhemoglobin, osmotic fragility, hemolysis, 2,3-diphosphoglycerate, cholinesterase, ATP, and antigen typing of treated cells were compared to controls. These pilot studies indicate that rGal could have potential biotechnical application in the production of universally transfusable red blood cells.

Original languageEnglish (US)
Pages (from-to)175-186
Number of pages12
JournalBiochemistry and Molecular Biology International
Volume46
Issue number1
StatePublished - 1998
Externally publishedYes

Fingerprint

Galactosidases
Soybeans
Glycine
Erythrocytes
Cells
Blood
Osmotic Fragility
Carboxyhemoglobin
2,3-Diphosphoglycerate
Methemoglobin
Erythrocyte Indices
Cholinesterases
Hemolysis
Galactose
Epitopes
Adenosine Triphosphate
Antigens
Seroconversion

Keywords

  • α-D-galactosidase
  • Blood type
  • Enzymatic conversion
  • Glycine max

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Vosnidou, N. C., Johnson, S. A., Mitra, M. M., Wells, D. C., Li, C. Q., Evans, M. L., ... Smith, D. (1998). Seroconversion of type B to O erythrocytes using recombinant Glycine max α-D-galactosidase. Biochemistry and Molecular Biology International, 46(1), 175-186.

Seroconversion of type B to O erythrocytes using recombinant Glycine max α-D-galactosidase. / Vosnidou, Nancy C.; Johnson, Scott A.; Mitra, Moon Moon; Wells, Donald C.; Li, Chun Qin; Evans, Maria L.; Harmata, Michael A.; Walker, John C.; Smith, Daniel.

In: Biochemistry and Molecular Biology International, Vol. 46, No. 1, 1998, p. 175-186.

Research output: Contribution to journalArticle

Vosnidou, NC, Johnson, SA, Mitra, MM, Wells, DC, Li, CQ, Evans, ML, Harmata, MA, Walker, JC & Smith, D 1998, 'Seroconversion of type B to O erythrocytes using recombinant Glycine max α-D-galactosidase', Biochemistry and Molecular Biology International, vol. 46, no. 1, pp. 175-186.
Vosnidou, Nancy C. ; Johnson, Scott A. ; Mitra, Moon Moon ; Wells, Donald C. ; Li, Chun Qin ; Evans, Maria L. ; Harmata, Michael A. ; Walker, John C. ; Smith, Daniel. / Seroconversion of type B to O erythrocytes using recombinant Glycine max α-D-galactosidase. In: Biochemistry and Molecular Biology International. 1998 ; Vol. 46, No. 1. pp. 175-186.
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