Seroconversion of type B to O erythrocytes using recombinant Glycine max α-D-galactosidase

Nancy C. Vosnidou, Scott A. Johnson, Moon Moon Mitra, Donald C. Wells, Chun Qin Li, Maria L. Evans, Michael A. Harmata, John C. Walker, Daniel S. Smith

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Recombinant α-D-galactosidase (rGal) from soybean (Glycine max) hydrolyzed the immunodominant α-D-galactose residue from the B epitope of red blood cells. This converted type B erythrocytes to type O which are 'universally' transfusable. Type B red blood cells were obtained from four different donors and enzymatically converted. Cell function parameters, including red cell indices, pH, methemoglobin, carboxyhemoglobin, osmotic fragility, hemolysis, 2,3-diphosphoglycerate, cholinesterase, ATP, and antigen typing of treated cells were compared to controls. These pilot studies indicate that rGal could have potential biotechnical application in the production of universally transfusable red blood cells.

Original languageEnglish (US)
Pages (from-to)175-186
Number of pages12
JournalBiochemistry and Molecular Biology International
Volume46
Issue number1
DOIs
StatePublished - 1998

Keywords

  • Blood type
  • Enzymatic conversion
  • Glycine max
  • α-D-galactosidase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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    Vosnidou, N. C., Johnson, S. A., Mitra, M. M., Wells, D. C., Li, C. Q., Evans, M. L., Harmata, M. A., Walker, J. C., & Smith, D. S. (1998). Seroconversion of type B to O erythrocytes using recombinant Glycine max α-D-galactosidase. Biochemistry and Molecular Biology International, 46(1), 175-186. https://doi.org/10.1080/15216549800203682