Serotonergic neurons in the alcohol preferring rats

F. C. Zhou, C. F. Pu, J. Murphy, L. Lumeng, T. K. Li

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Previously, we have shown that the serotonin (5-HT) content and fiber density in a number of terminal brain regions have been found to be decreased in the selectively bred, alcohol preferring (P) rats than in the alcohol nonpreferring (NP) rats. In this study, we further report that, compared with NP rats, there were fewer 5-HT-immunostained (5-HT-IM) neurons in the major ascending raphe nuclei of the P rats. Among the three major groups of 5-HT neurons responsible for the majority of ascending projections to forebrain, dorsal raphe (B7), median raphe (B8), and B9, there were fewer 5-HT-IM neurons in the median and dorsal raphe (not including nucleus oralis) of P rats, compared with NP rats (unpaired Student's test). No difference was observed in the B9 group. When the animals were treated with pargyline and L-tryptophan to enhance the 5-HT in the neurons, the number of 5-HT-IM neurons increased in both lines of rats. However, the difference in the number of 5-HT neurons between the rat lines remained. The intensity of 5-HT-IM was also found to be lower in the dorsal raphe neurons of the untreated P than in the untreated NP rats. The decreased 5-HT-IM was supported by high performance liquid chromatography measurement of 5-HT content, which also indicated that 5-HT content of the dorsal raphe was lower in the P than in the NP rats. These results indicate that lower 5-HT content and fewer 5-HT-IM neurons in the raphe account for the reduced density of detectable 5-HT-IM fibers in the terminal brain regions in the P rats, and that 5-HT neuronal transmission is reduced in the alcohol preferring rats.

Original languageEnglish (US)
Pages (from-to)397-403
Number of pages7
JournalAlcohol
Volume11
Issue number5
DOIs
StatePublished - Jan 1 1994

Keywords

  • 5-HT
  • Alcoholism
  • Animal model
  • Image analysis
  • Immunocytochemistry
  • Raphe
  • Serotonin
  • Serotonin neurons

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Neuroscience(all)
  • Behavioral Neuroscience
  • Toxicology
  • Health(social science)

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  • Cite this

    Zhou, F. C., Pu, C. F., Murphy, J., Lumeng, L., & Li, T. K. (1994). Serotonergic neurons in the alcohol preferring rats. Alcohol, 11(5), 397-403. https://doi.org/10.1016/0741-8329(94)90024-8