Serotonin-2 receptors in the CNS of alcohol-preferring and -nonpreferring rats

W. J. McBride, E. Chernet, J. A. Rabold, L. Lumeng, T. K. Li

Research output: Contribution to journalArticle

56 Scopus citations


The densities of serotonin-2(5-HT2) receptors, labelled with [3H]ketanserin (Ket), were examined in the CNS of alcohol-naive, adult, male alcohol-preferring (P) and nonpreferring (NP) rats using quantitative autoradiography. The densities of binding sites labelled with 2.0 nM [3H]Ket were 15-25% lower (p < 0.05) in layer IV of the medial prefrontal, frontal, cingulate, parietal, and temporal cortices of the P line compared with the NP line. [3H]Ket binding in the P rats was also 40-50% lower (p < 0.05) in the medial and lateral nucleus accumbens, olfactory tubercle, and caudate-putamen, and 20% lower (p < 0.05) in the claustrum, compared with the NP line. No differences in 2.0 nM [3H]Ket binding were observed between the lines in the piriform cortex, hypothalamus, amygdala, hippocampus, ventral tegmental area, substantia nigra, central gray, and interpenduncular nucleus. Scatchard analysis of [3H]Ket binding (0.5-6.0 nM), determined in layer IV of the frontal and parietal cortex and in the lateral nucleus accumbens, indicated lower Bmax values without a change in Kd for the P compared to the NP rats. The results of this study indicate that there are fewer 5-HT2 receptors in certain CNS regions of the P relative to the NP rats.

Original languageEnglish (US)
Pages (from-to)631-636
Number of pages6
JournalPharmacology, Biochemistry and Behavior
Issue number3
StatePublished - Nov 1993


  • Alcohol-nonpreferring rats
  • Alcohol-preferring rats
  • Autoradiography
  • CNS
  • Ketanserin binding
  • Serotonin-2 receptors

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

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