Serotonin-2 receptors in the CNS of alcohol-preferring and -nonpreferring rats

W. J. McBride, E. Chernet, J. A. Rabold, L. Lumeng, T. K. Li

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The densities of serotonin-2(5-HT2) receptors, labelled with [3H]ketanserin (Ket), were examined in the CNS of alcohol-naive, adult, male alcohol-preferring (P) and nonpreferring (NP) rats using quantitative autoradiography. The densities of binding sites labelled with 2.0 nM [3H]Ket were 15-25% lower (p < 0.05) in layer IV of the medial prefrontal, frontal, cingulate, parietal, and temporal cortices of the P line compared with the NP line. [3H]Ket binding in the P rats was also 40-50% lower (p < 0.05) in the medial and lateral nucleus accumbens, olfactory tubercle, and caudate-putamen, and 20% lower (p < 0.05) in the claustrum, compared with the NP line. No differences in 2.0 nM [3H]Ket binding were observed between the lines in the piriform cortex, hypothalamus, amygdala, hippocampus, ventral tegmental area, substantia nigra, central gray, and interpenduncular nucleus. Scatchard analysis of [3H]Ket binding (0.5-6.0 nM), determined in layer IV of the frontal and parietal cortex and in the lateral nucleus accumbens, indicated lower Bmax values without a change in Kd for the P compared to the NP rats. The results of this study indicate that there are fewer 5-HT2 receptors in certain CNS regions of the P relative to the NP rats.

Original languageEnglish
Pages (from-to)631-636
Number of pages6
JournalPharmacology Biochemistry and Behavior
Volume46
Issue number3
DOIs
StatePublished - 1993

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Serotonin 5-HT2 Receptors
Ketanserin
Rats
Alcohols
Parietal Lobe
Nucleus Accumbens
Frontal Lobe
Ventral Tegmental Area
Putamen
Gyrus Cinguli
Substantia Nigra
Temporal Lobe
Amygdala
Basal Ganglia
Prefrontal Cortex
Autoradiography
Hypothalamus
Hippocampus
Serotonin
Binding Sites

Keywords

  • Alcohol-nonpreferring rats
  • Alcohol-preferring rats
  • Autoradiography
  • CNS
  • Ketanserin binding
  • Serotonin-2 receptors

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Serotonin-2 receptors in the CNS of alcohol-preferring and -nonpreferring rats. / McBride, W. J.; Chernet, E.; Rabold, J. A.; Lumeng, L.; Li, T. K.

In: Pharmacology Biochemistry and Behavior, Vol. 46, No. 3, 1993, p. 631-636.

Research output: Contribution to journalArticle

McBride, W. J. ; Chernet, E. ; Rabold, J. A. ; Lumeng, L. ; Li, T. K. / Serotonin-2 receptors in the CNS of alcohol-preferring and -nonpreferring rats. In: Pharmacology Biochemistry and Behavior. 1993 ; Vol. 46, No. 3. pp. 631-636.
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