Serotonin-3 receptor and ethanol-stimulated somatodendritic dopamine release

A. D. Campbell, R. R. Kohl, W. J. McBride

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

The effects of local application of the 5-HT3 receptor agonist, 1-(m- chlorophenyl)-biguanide (CPBG), and IP administration of ethanol on the extracellular levels of dopamine (DA) in the ventral tegmental area (VTA) were studied using in vivo microdialysis. Adult female Wistar rats were implanted with microdialysis probes in the VTA at least 24 h before each experiment. Stable extracellular levels of DA (101 ± 9 fmol/20 min) were established before initiating the experiments. Application of 10-250 μM CPBG through the microdialysis probe dose-dependent enhanced the extracellular concentrations of DA but did not alter the levels of either 3,4- dihydroxyphenylacetic acid or homovanillic acid in the dialysate. The effects of CPBG were reversible and dependent upon Ca2+. Co-perfusion with the 5- HT3 receptor antagonist, 3-tropanyl-indole-3-carboxylate (ICS 205-930), inhibited the effects of CPBG on enhancing extracellular DA levels. The IP administration of 2 g/kg ethanol significantly (p < 0.005) enhanced the levels of DA to 150% of baseline values; this ethanol-induced increase was prevented by local perfusion with 100 μM ICS 205-930. These results suggest that 5-HT3 receptors in the VTA are involved in regulating the somatodendritic release of DA and in mediating the stimulatory effects of ethanol on this neuronal system.

Original languageEnglish (US)
Pages (from-to)569-574
Number of pages6
JournalAlcohol
Volume13
Issue number6
DOIs
StatePublished - Nov 1 1996

Keywords

  • 1-(m-Chlorophenyl)-biguanide
  • Ethanol- stimulated dopamine release
  • ICS 205-930
  • In vivo microdialysis
  • Serotonin-3 receptor
  • Somatodendritic dopamine release
  • Ventral tegmental area

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neuroscience(all)
  • Toxicology
  • Health(social science)

Fingerprint Dive into the research topics of 'Serotonin-3 receptor and ethanol-stimulated somatodendritic dopamine release'. Together they form a unique fingerprint.

  • Cite this