Serotonin concentration and turnover in cerebelum and other brain regions of pcd mutant mice

Bernardino Ghetti, Kenneth W. Perry, Ray W. Fuller

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The Purkinje cell degeneration (pcd) mutant mouse is characterized by loss of Purkinje cells in the cerebellum. Loss of granule cells occurs and is severe in pcd mutants after 9 months of age. Since Purkinje cells and granule cells represent two groups of target cells for serotonin neurons projecting from raphe nuclei and other brain areas, the content and turnover of serotonin in the cerebellum were determined in pcd mice aged 3-15 months. The content of serotonin was not decreased in pcd mouse cerebellum but tended to be slightly increased after 7 months. The ratio of 5-hydroxyindoleacetic acid (5-HIAA) to serotonin was significantly decreased in cerebellum at 7-15 months but not at 3 or 6 months. The decrease in this ratio is indicative of decreased serotonin turnover. Similar changes were not seen in brainstem or hypothalamus in mice up to 14 months old, but slight decreases were observed at 15 months. Another index of turnover, the accumulation of 5-HIAA after administration of probenecid to block its efflux from brain, was decreased by 46% in 7-month-old pcd mice in the cerebellum but not in the brainstem or hypothalamus. The decrease in serotonin turnover in pcd mouse cerebellum occurs subsequent to and perhaps due to the loss of the target Purkinje and granule cells.

Original languageEnglish (US)
Pages (from-to)367-371
Number of pages5
JournalBrain research
Volume458
Issue number2
DOIs
StatePublished - Aug 23 1988

Keywords

  • 5-Hydroxyindoleacetic acid
  • Cerebellum
  • Mutant mouse pcd
  • Purkinje cell
  • Serotonin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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