Serotonin-immunoreactivity in the cerebellum of two neurological mutant mice and the corresponding wild-type genetic stocks

Lazaros C. Triarhou, Bernardino Ghetti

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Using a serotonin (5-HT)-specific antibody, we examined the 5-HTergic innervation of the cerebellum in the normal mouse ( + +) and in two neurological mutants: weaver (wv/wv), which are characterized by a genetically determined loss of granule cells, and 'Purkinje cell degeneration' (pcd/pcd), which are characterized by a genetically determined loss of Purkinje cells. In normal cerebellum, serotonin-immunoreactive (5-HT-ir) fibers are discrete and ascend to all three layers of the cerebellar cortex. Serotonin-immunoreactive fibers have a much higher density in the atrophic cerebella of both weaver and pcd mutants, where they form multidirectional contours. These anatomical findings provide a profile of 5-HT axon innervation of mouse cerebellum and extend previous neurochemical observations on the metabolic state of cerebellar 5-HT in neurological mutants.

Original languageEnglish
Pages (from-to)421-428
Number of pages8
JournalJournal of Chemical Neuroanatomy
Volume4
Issue number6
DOIs
StatePublished - 1991

Fingerprint

Neurologic Mutant Mice
Cerebellum
Serotonin
Purkinje Cells
Cerebellar Cortex
Axons
Antibodies

Keywords

  • Immunocytochemistry
  • Mouse
  • Purkinje cell degeneration
  • Weaver

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

@article{f6adbfbf87fd462283bf6705a84524ce,
title = "Serotonin-immunoreactivity in the cerebellum of two neurological mutant mice and the corresponding wild-type genetic stocks",
abstract = "Using a serotonin (5-HT)-specific antibody, we examined the 5-HTergic innervation of the cerebellum in the normal mouse ( + +) and in two neurological mutants: weaver (wv/wv), which are characterized by a genetically determined loss of granule cells, and 'Purkinje cell degeneration' (pcd/pcd), which are characterized by a genetically determined loss of Purkinje cells. In normal cerebellum, serotonin-immunoreactive (5-HT-ir) fibers are discrete and ascend to all three layers of the cerebellar cortex. Serotonin-immunoreactive fibers have a much higher density in the atrophic cerebella of both weaver and pcd mutants, where they form multidirectional contours. These anatomical findings provide a profile of 5-HT axon innervation of mouse cerebellum and extend previous neurochemical observations on the metabolic state of cerebellar 5-HT in neurological mutants.",
keywords = "Immunocytochemistry, Mouse, Purkinje cell degeneration, Weaver",
author = "Triarhou, {Lazaros C.} and Bernardino Ghetti",
year = "1991",
doi = "10.1016/0891-0618(91)90022-5",
language = "English",
volume = "4",
pages = "421--428",
journal = "Journal of Chemical Neuroanatomy",
issn = "0891-0618",
publisher = "Elsevier",
number = "6",

}

TY - JOUR

T1 - Serotonin-immunoreactivity in the cerebellum of two neurological mutant mice and the corresponding wild-type genetic stocks

AU - Triarhou, Lazaros C.

AU - Ghetti, Bernardino

PY - 1991

Y1 - 1991

N2 - Using a serotonin (5-HT)-specific antibody, we examined the 5-HTergic innervation of the cerebellum in the normal mouse ( + +) and in two neurological mutants: weaver (wv/wv), which are characterized by a genetically determined loss of granule cells, and 'Purkinje cell degeneration' (pcd/pcd), which are characterized by a genetically determined loss of Purkinje cells. In normal cerebellum, serotonin-immunoreactive (5-HT-ir) fibers are discrete and ascend to all three layers of the cerebellar cortex. Serotonin-immunoreactive fibers have a much higher density in the atrophic cerebella of both weaver and pcd mutants, where they form multidirectional contours. These anatomical findings provide a profile of 5-HT axon innervation of mouse cerebellum and extend previous neurochemical observations on the metabolic state of cerebellar 5-HT in neurological mutants.

AB - Using a serotonin (5-HT)-specific antibody, we examined the 5-HTergic innervation of the cerebellum in the normal mouse ( + +) and in two neurological mutants: weaver (wv/wv), which are characterized by a genetically determined loss of granule cells, and 'Purkinje cell degeneration' (pcd/pcd), which are characterized by a genetically determined loss of Purkinje cells. In normal cerebellum, serotonin-immunoreactive (5-HT-ir) fibers are discrete and ascend to all three layers of the cerebellar cortex. Serotonin-immunoreactive fibers have a much higher density in the atrophic cerebella of both weaver and pcd mutants, where they form multidirectional contours. These anatomical findings provide a profile of 5-HT axon innervation of mouse cerebellum and extend previous neurochemical observations on the metabolic state of cerebellar 5-HT in neurological mutants.

KW - Immunocytochemistry

KW - Mouse

KW - Purkinje cell degeneration

KW - Weaver

UR - http://www.scopus.com/inward/record.url?scp=0025954832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025954832&partnerID=8YFLogxK

U2 - 10.1016/0891-0618(91)90022-5

DO - 10.1016/0891-0618(91)90022-5

M3 - Article

C2 - 1781951

AN - SCOPUS:0025954832

VL - 4

SP - 421

EP - 428

JO - Journal of Chemical Neuroanatomy

JF - Journal of Chemical Neuroanatomy

SN - 0891-0618

IS - 6

ER -