Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies

W. J. Newhall, P. Terho, C. E. Wilde, Byron Batteiger, R. B. Jones

Research output: Contribution to journalArticle

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Abstract

A panel of 15 monoclonal antibodies was prepared that could distinguish among the 15 serovars of Chlamydia trachomatis. Twelve of these antibodies were specific for a single serovar (A, B, C, D, E, F, G, H, I, K, L1, and L2) and three were specific for two serovars (B/Ba, C/J, and C/L3). Ten of the serovar-specific and two of the bispecific antibodies were shown by immunoblotting to recognize epitopes on the major outer membrane protein. These data provide evidence that such epitopes are closely correlated with and may be partly responsible for the antigenic variations detected by microimmunofluorescence that distinguish the currently recognized serovars. When used in a radioimmunoassay, these antibodies correctly identified the serovar of 17 strains that had been serotyped by the microimmunofluorescence test. In addition, we found that the chlamydial antigen derived from 1.0 cm2 of an infected HeLa cell monolayer was sufficient to allow serotyping with these antibodies. Thus, these monoclonal antibodies may provide a rapid and reliable alternative to mouse immunization and microimmunofluorescence for serotyping of clinical isolates.

Original languageEnglish (US)
Pages (from-to)333-338
Number of pages6
JournalJournal of Clinical Microbiology
Volume23
Issue number2
StatePublished - 1986
Externally publishedYes

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Chlamydia trachomatis
Monoclonal Antibodies
Serotyping
Antibodies
Epitopes
Bispecific Antibodies
Antigenic Variation
HeLa Cells
Immunoblotting
Radioimmunoassay
Serogroup
Immunization
Membrane Proteins
Antigens

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies. / Newhall, W. J.; Terho, P.; Wilde, C. E.; Batteiger, Byron; Jones, R. B.

In: Journal of Clinical Microbiology, Vol. 23, No. 2, 1986, p. 333-338.

Research output: Contribution to journalArticle

Newhall, W. J. ; Terho, P. ; Wilde, C. E. ; Batteiger, Byron ; Jones, R. B. / Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies. In: Journal of Clinical Microbiology. 1986 ; Vol. 23, No. 2. pp. 333-338.
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