Serum-free recombinant production of adenovirus using a hollow fiber capillary system

Thomas Gardner, S. C. Ko, L. Yang, J. J S Cadwell, L. W K Chung, Chinghai Kao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.

Original languageEnglish (US)
Pages (from-to)422-427
Number of pages6
JournalBioTechniques
Volume30
Issue number2
StatePublished - 2001
Externally publishedYes

Fingerprint

Adenoviridae
Cell culture
Fibers
Serum
Cell Culture Techniques
Purification
Culture Media
Serum-Free Culture Media
Acoustic waves
Clinical Trials
Research

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gardner, T., Ko, S. C., Yang, L., Cadwell, J. J. S., Chung, L. W. K., & Kao, C. (2001). Serum-free recombinant production of adenovirus using a hollow fiber capillary system. BioTechniques, 30(2), 422-427.

Serum-free recombinant production of adenovirus using a hollow fiber capillary system. / Gardner, Thomas; Ko, S. C.; Yang, L.; Cadwell, J. J S; Chung, L. W K; Kao, Chinghai.

In: BioTechniques, Vol. 30, No. 2, 2001, p. 422-427.

Research output: Contribution to journalArticle

Gardner, T, Ko, SC, Yang, L, Cadwell, JJS, Chung, LWK & Kao, C 2001, 'Serum-free recombinant production of adenovirus using a hollow fiber capillary system', BioTechniques, vol. 30, no. 2, pp. 422-427.
Gardner, Thomas ; Ko, S. C. ; Yang, L. ; Cadwell, J. J S ; Chung, L. W K ; Kao, Chinghai. / Serum-free recombinant production of adenovirus using a hollow fiber capillary system. In: BioTechniques. 2001 ; Vol. 30, No. 2. pp. 422-427.
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