Serum-free recombinant production of adenovirus using a hollow fiber capillary system

T. A. Gardner, S. C. Ko, L. Yang, J. J.S. Cadwell, L. W.K. Chung, C. Kao

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.

Original languageEnglish (US)
Pages (from-to)422-427
Number of pages6
JournalBioTechniques
Volume30
Issue number2
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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    Gardner, T. A., Ko, S. C., Yang, L., Cadwell, J. J. S., Chung, L. W. K., & Kao, C. (2001). Serum-free recombinant production of adenovirus using a hollow fiber capillary system. BioTechniques, 30(2), 422-427. https://doi.org/10.2144/01302pf01