Serum miR-204 is an early biomarker of type 1 diabetes-associated pancreatic beta-cell loss

Guanlan Xu, Lance A. Thielen, Junqin Chen, Truman B. Grayson, Tiffany Grimes, S. Louis Bridges, Hubert M. Tse, Blair Smith, Rakesh Patel, Peng Li, Carmella Evans-Molina, Fernando Ovalle, Anath Shalev

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Pancreatic beta-cell death is a major factor in the pathogenesis of type 1 diabetes (T1D), but straightforward methods to measure beta-cell loss in humans are lacking, underlining the need for novel biomarkers. Using studies in INS-1 cells, human islets, diabetic mice, and serum samples of subjects with T1D at different stages, we have identified serum miR-204 as an early biomarker of T1D-associated beta-cell loss in humans. MiR-204 is a highly enriched microRNA in human beta-cells, and we found that it is released from dying beta-cells and detectable in human serum. We further discovered that serum miR-204 was elevated in children and adults with T1D and in autoantibody-positive at-risk subjects but not in type 2 diabetes or other autoimmune diseases and was inversely correlated with remaining beta-cell function in recent-onset T1D. Thus, serum miR-204 may provide a much needed novel approach to assess early T1D-associated human beta-cell loss even before onset of overt disease.

Original languageEnglish (US)
Pages (from-to)E723-E730
JournalAmerican journal of physiology. Endocrinology and metabolism
Volume317
Issue number4
DOIs
StatePublished - Oct 1 2019

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Keywords

  • T1D
  • beta cell death
  • biomarker
  • miR-204

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Xu, G., Thielen, L. A., Chen, J., Grayson, T. B., Grimes, T., Bridges, S. L., Tse, H. M., Smith, B., Patel, R., Li, P., Evans-Molina, C., Ovalle, F., & Shalev, A. (2019). Serum miR-204 is an early biomarker of type 1 diabetes-associated pancreatic beta-cell loss. American journal of physiology. Endocrinology and metabolism, 317(4), E723-E730. https://doi.org/10.1152/ajpendo.00122.2019