Serum miR-204 is an early biomarker of type 1 diabetes-associated pancreatic beta-cell loss

Guanlan Xu, Lance A. Thielen, Junqin Chen, Truman B. Grayson, Tiffany Grimes, S. Louis Bridges, Hubert M. Tse, Blair Smith, Rakesh Patel, Peng Li, Carmella Evans-Molina, Fernando Ovalle, Anath Shalev

Research output: Contribution to journalArticle

Abstract

Pancreatic beta-cell death is a major factor in the pathogenesis of type 1 diabetes (T1D), but straightforward methods to measure beta-cell loss in humans are lacking, underlining the need for novel biomarkers. Using studies in INS-1 cells, human islets, diabetic mice, and serum samples of subjects with T1D at different stages, we have identified serum miR-204 as an early biomarker of T1D-associated beta-cell loss in humans. MiR-204 is a highly enriched microRNA in human beta-cells, and we found that it is released from dying beta-cells and detectable in human serum. We further discovered that serum miR-204 was elevated in children and adults with T1D and in autoantibody-positive at-risk subjects but not in type 2 diabetes or other autoimmune diseases and was inversely correlated with remaining beta-cell function in recent-onset T1D. Thus, serum miR-204 may provide a much needed novel approach to assess early T1D-associated human beta-cell loss even before onset of overt disease.

Original languageEnglish (US)
Pages (from-to)E723-E730
JournalAmerican journal of physiology. Endocrinology and metabolism
Volume317
Issue number4
DOIs
StatePublished - Oct 1 2019

Fingerprint

Insulin-Secreting Cells
Type 1 Diabetes Mellitus
Biomarkers
Serum
MicroRNAs
Islets of Langerhans
Autoantibodies
Type 2 Diabetes Mellitus
Autoimmune Diseases
Cell Death

Keywords

  • beta cell death
  • biomarker
  • miR-204
  • T1D

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Serum miR-204 is an early biomarker of type 1 diabetes-associated pancreatic beta-cell loss. / Xu, Guanlan; Thielen, Lance A.; Chen, Junqin; Grayson, Truman B.; Grimes, Tiffany; Bridges, S. Louis; Tse, Hubert M.; Smith, Blair; Patel, Rakesh; Li, Peng; Evans-Molina, Carmella; Ovalle, Fernando; Shalev, Anath.

In: American journal of physiology. Endocrinology and metabolism, Vol. 317, No. 4, 01.10.2019, p. E723-E730.

Research output: Contribution to journalArticle

Xu, G, Thielen, LA, Chen, J, Grayson, TB, Grimes, T, Bridges, SL, Tse, HM, Smith, B, Patel, R, Li, P, Evans-Molina, C, Ovalle, F & Shalev, A 2019, 'Serum miR-204 is an early biomarker of type 1 diabetes-associated pancreatic beta-cell loss', American journal of physiology. Endocrinology and metabolism, vol. 317, no. 4, pp. E723-E730. https://doi.org/10.1152/ajpendo.00122.2019
Xu, Guanlan ; Thielen, Lance A. ; Chen, Junqin ; Grayson, Truman B. ; Grimes, Tiffany ; Bridges, S. Louis ; Tse, Hubert M. ; Smith, Blair ; Patel, Rakesh ; Li, Peng ; Evans-Molina, Carmella ; Ovalle, Fernando ; Shalev, Anath. / Serum miR-204 is an early biomarker of type 1 diabetes-associated pancreatic beta-cell loss. In: American journal of physiology. Endocrinology and metabolism. 2019 ; Vol. 317, No. 4. pp. E723-E730.
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