Seven Transmembrane Domain Receptor Subtypes Identified in NG108-15 Cells by Reverse Transcription-Polymerase Chain Reaction

Rebecca Chan, A. W. Mcbride, David Crabb

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

NG108-15 neuroblastoma x glioma cells are widely used for the study of neurotransmitter receptors. We utilized reverse transcription-polymerase chain reaction to amplify members of the seven transmembrane domain class of G-protein linked receptors using RNA isolated from NG108-15 cells. Two complementary DNAs representing receptors were obtained; based upon comparison with the sequence database, they probably represent the murine dopamine D1A receptor and a receptor closely related to the serotonin 5HT1D receptor subtype. The finding of the 5HT receptor subtype is of interest, as only the 5HT3 subtype was previously identified in NG108-15 cells by pharmacological means. Certain responses of NG108-15 cells to serotonin have been described that do not appear to be mediated by known 5HT receptor subtypes. The cDNA we cloned may therefore represent an additional 5HT1D subclass.

Original languageEnglish
Pages (from-to)1311-1317
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume205
Issue number2
DOIs
StatePublished - Dec 15 1994

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Polymerase chain reaction
Transcription
Reverse Transcription
Complementary DNA
Polymerase Chain Reaction
Neurotransmitter Receptor
Serotonin Receptors
GTP-Binding Proteins
Serotonin
RNA
Neuroblastoma
Glioma
Databases
Pharmacology
dopamine D1A receptor
DNA receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

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