Sex Differences in Chondrocyte Maturation in the Mandibular Condyle from a Decreased Occlusal Loading Model

J. Chen, T. Sobue, Achint Utreja, Z. Kalajzic, M. Xu, T. Kilts, M. Young, S. Wadhwa

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Temporomandibular joint disorders (TMDs) predominantly afflict women of childbearing age. Defects in mechanical loading-induced temporomandibular joint (TMJ) remodeling are believed to be a major etiological factor in the development of TMD. The goal of this study was to determine if there are sex differences in CD-1 and C57BL/6 mice exposed to a decreased occlusal loading TMJ remodeling model. Male and female CD-1 and C57BL/6 mice, 21 days old, were each divided into two groups. They were fed either a normal pellet diet (normal loading) or a soft diet and had their incisors trimmed out of occlusion (decreased occlusal loading) for 4 weeks. The mandibular condylar cartilage was evaluated by histology, and the subchondral bone was evaluated by micro-CT analysis. Gene expression from both was evaluated by real-time PCR analysis. In both strains and sexes of mice, decreased occlusal loading caused similar effects in the subchondral bone, decreases in bone volume and total volume compared with their normal loading controls. However, in both strains, decreased occlusal loading caused a significant decrease in the expression of collagen type II (Col2) and Sox9 only in female mice, but not in male mice, compared with their normal loading controls. Decreased occlusal loading causes decreased bone volume in both sexes and a decrease in early chondrocyte maturation exclusively in female mice.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalCalcified Tissue International
Volume89
Issue number2
DOIs
StatePublished - Aug 2011
Externally publishedYes

Fingerprint

Mandibular Condyle
Chondrocytes
Sex Characteristics
Bone and Bones
Temporomandibular Joint Disorders
Temporomandibular Joint
Inbred C57BL Mouse
Diet
Collagen Type II
Incisor
Cartilage
Real-Time Polymerase Chain Reaction
Histology
Gene Expression

Keywords

  • Fibrocartilage
  • Mechanical loading
  • Mouse model
  • Subchondral bone
  • Temporomandibular joint

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Sex Differences in Chondrocyte Maturation in the Mandibular Condyle from a Decreased Occlusal Loading Model. / Chen, J.; Sobue, T.; Utreja, Achint; Kalajzic, Z.; Xu, M.; Kilts, T.; Young, M.; Wadhwa, S.

In: Calcified Tissue International, Vol. 89, No. 2, 08.2011, p. 123-129.

Research output: Contribution to journalArticle

Chen, J, Sobue, T, Utreja, A, Kalajzic, Z, Xu, M, Kilts, T, Young, M & Wadhwa, S 2011, 'Sex Differences in Chondrocyte Maturation in the Mandibular Condyle from a Decreased Occlusal Loading Model', Calcified Tissue International, vol. 89, no. 2, pp. 123-129. https://doi.org/10.1007/s00223-011-9498-9
Chen, J. ; Sobue, T. ; Utreja, Achint ; Kalajzic, Z. ; Xu, M. ; Kilts, T. ; Young, M. ; Wadhwa, S. / Sex Differences in Chondrocyte Maturation in the Mandibular Condyle from a Decreased Occlusal Loading Model. In: Calcified Tissue International. 2011 ; Vol. 89, No. 2. pp. 123-129.
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