Sex dimorphisms in activated mesenchymal stem cell function

Paul R. Crisostomo, Meijing Wang, Christine M. Herring, Eric D. Morrell, Preethi Seshadri, Kirstan K. Meldrum, Daniel R. Meldrum

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The plasticity of bone marrow-derived stem cells (BMSCs) has resulted in positive remodeling and the regeneration of viable tissues. However, BMSC release of growth factors, which limit apoptosis and inflammation, may play an important role in conferring organ protection. Recent studies also indicate that those patients with higher circulating BMSC counts may be more resistant to septic and traumatic insults. There are clear sex differences in response to such insults. Within the population of BMSC, mesenchymal stem cells (MSCs) may have clinical advantages. Therefore, we hypothesize that sex differences in the MSC paracrine response to acute injury exist.Mesenchymal stem cells were obtained from male and female mice. One million MSCs per well (triplicate wells per group) were stressed by hypoxia and increasing doses of endotoxin (lipopolysaccharide [LPS]) and hydrogen peroxide. Mesenchymal stem cell activation was determined by measuring vascular endothelial growth factor (VEGF) and tumor necrosis factor α production by enzyme-linked immunosorbent assay. Differences were considered significant if P < 0.05. RESULTS: Lipopolysaccharide resulted in significant activation of both male and female MSCs. However, LPS provoked significantly more VEGF production in female MSCs versus male MSCs at all LPS doses. Hypoxia of 1 h and hydrogen pyroxide exposure also caused significantly more VEGF production in female MSCs versus male MSCs. Female MSCs expressed significantly less tumor necrosis factor α than male MSCs after acute LPS and hypoxia. CONCLUSION: This study constitutes the first demonstration that sex differences exist in activated MSC function. Sex differences in progenitor cell function may have important implications in understanding the observed sex differences in the host's response to injury.

Original languageEnglish
Pages (from-to)571-574
Number of pages4
JournalShock
Volume26
Issue number6
DOIs
StatePublished - Dec 2006

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Mesenchymal Stromal Cells
Sex Characteristics
Lipopolysaccharides
Stem Cells
Bone Marrow
Vascular Endothelial Growth Factor A
Tumor Necrosis Factor-alpha
Wounds and Injuries
Endotoxins
Hydrogen Peroxide
Regeneration
Hydrogen
Intercellular Signaling Peptides and Proteins
Cell Count
Enzyme-Linked Immunosorbent Assay
Apoptosis
Inflammation

Keywords

  • MSC
  • TNF
  • VEGF

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Crisostomo, P. R., Wang, M., Herring, C. M., Morrell, E. D., Seshadri, P., Meldrum, K. K., & Meldrum, D. R. (2006). Sex dimorphisms in activated mesenchymal stem cell function. Shock, 26(6), 571-574. https://doi.org/10.1097/01.shk.0000233195.63859.ef

Sex dimorphisms in activated mesenchymal stem cell function. / Crisostomo, Paul R.; Wang, Meijing; Herring, Christine M.; Morrell, Eric D.; Seshadri, Preethi; Meldrum, Kirstan K.; Meldrum, Daniel R.

In: Shock, Vol. 26, No. 6, 12.2006, p. 571-574.

Research output: Contribution to journalArticle

Crisostomo, PR, Wang, M, Herring, CM, Morrell, ED, Seshadri, P, Meldrum, KK & Meldrum, DR 2006, 'Sex dimorphisms in activated mesenchymal stem cell function', Shock, vol. 26, no. 6, pp. 571-574. https://doi.org/10.1097/01.shk.0000233195.63859.ef
Crisostomo PR, Wang M, Herring CM, Morrell ED, Seshadri P, Meldrum KK et al. Sex dimorphisms in activated mesenchymal stem cell function. Shock. 2006 Dec;26(6):571-574. https://doi.org/10.1097/01.shk.0000233195.63859.ef
Crisostomo, Paul R. ; Wang, Meijing ; Herring, Christine M. ; Morrell, Eric D. ; Seshadri, Preethi ; Meldrum, Kirstan K. ; Meldrum, Daniel R. / Sex dimorphisms in activated mesenchymal stem cell function. In: Shock. 2006 ; Vol. 26, No. 6. pp. 571-574.
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