SH3 domain-containing protein 2 plays a crucial role at the step of membrane tubulation during cell plate formation

Gyeongik Ahn, Hyeran Kim, Dae Heon Kim, Hong Hanh, Youngdae Yoon, Indira Singaram, Kaveesha J. Wijesinghe, Kristen A. Johnson, Xiaohong Zhuang, Zizhen Liang, Robert V. Stahelin, Liwen Jiang, Wonhwa Cho, Byung Ho Kang, Inhwan Hwang

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

During cytokinesis in plants, trans-Golgi network-derived vesicles accumulate at the center of dividing cells and undergo various structural changes to give rise to the planar cell plate. However, how this conversion occurs at the molecular level remains elusive. In this study, we report that SH3 Domain-Containing Protein 2 (SH3P2) in Arabidopsis thaliana plays a crucial role in converting vesicles to the planar cell plate. SH3P2 RNAi plants showed cytokinesis-defective phenotypes and produced aggregations of vesicles at the leading edge of the cell plate. SH3P2 localized to the leading edge of the cell plate, particularly the constricted or curved regions of the cell plate. The BAR domain of SH3P2 induced tubulation of vesicles. SH3P2 formed a complex with dynamin-related protein 1A (DRP1A) and affected DRP1A accumulation to the cell plate. Based on these results, we propose that SH3P2 functions together with DRP1A to convert the fused vesicles to tubular structures during cytokinesis.

Original languageEnglish (US)
Pages (from-to)1388-1405
Number of pages18
JournalPlant Cell
Volume29
Issue number6
DOIs
StatePublished - Jun 2017

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'SH3 domain-containing protein 2 plays a crucial role at the step of membrane tubulation during cell plate formation'. Together they form a unique fingerprint.

  • Cite this

    Ahn, G., Kim, H., Kim, D. H., Hanh, H., Yoon, Y., Singaram, I., Wijesinghe, K. J., Johnson, K. A., Zhuang, X., Liang, Z., Stahelin, R. V., Jiang, L., Cho, W., Kang, B. H., & Hwang, I. (2017). SH3 domain-containing protein 2 plays a crucial role at the step of membrane tubulation during cell plate formation. Plant Cell, 29(6), 1388-1405. https://doi.org/10.1105/tpc.17.00108