Shear stress-mediated vascular growth

D. A. Tails, S. D. Schriver, J. L. Unthank, R. L. Prewitt

Research output: Contribution to journalArticle

Abstract

In order to characterize in vivo shear stress-mediated vascular growth, adjacent rat mesenteric ileal and second-order arterial branches were ligated to induce blood flow increases through the immediately adjacent ileal artery. At one, three, and seven days animals were sacrificed and the mesenteric vasculature was perfusion-fixed and paraffin-embedded. Arterial cross-sections were cut and stained with toluidine blue for morphological assessment, and immunocytochemistry (ICC) was performed for proliferating cell nuclear antigen to measure DNA replication. Results indicate that, while arterial pressures were not altered by the ligation, ileal artery blood flow and shear stress were increased approximately 200% initially after ligation. In arteries with elevated flow, mean lumen diameter and wall area increased significantly compared to same animal control vessels: ileal artery at seven days (255 ± 12 vs. 287 ± 12 μm; 11394 ± 1137 vs. 18016 ± 2648 um2, respectively), and second-order artery at three (188 ± 8 vs. 215 ± 9 μm; 7263 ± 591 vs. 11073 ± 1254 μm2, respectively) and seven (201 ± 22 vs. 277 ± 23 μm; 5883 ± 676 vs. 12936 ± 1684 μm2, respectively) days. ICC data suggest an initial medial hypertrophy in the larger vessel followed by medial hyperplasia, while the smaller vessel initially exhibits medial hyperplasia with subsequent medial cellular hypertrophy These data suggest that flowmediated vascular growth occurs through a combination of cellular hypertrophy and hyperplasia and is dependent on the order of the mesenteric arterial branch.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

blood vessels
arteries
shear stress
Blood Vessels
Shear stress
Animals
Blood
Arteries
Tolonium Chloride
hypertrophy
hyperplasia
Hypertrophy
Proliferating Cell Nuclear Antigen
Hyperplasia
Growth
Plastic flow
Paraffin
immunocytochemistry
Rats
blood flow

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Tails, D. A., Schriver, S. D., Unthank, J. L., & Prewitt, R. L. (1996). Shear stress-mediated vascular growth. FASEB Journal, 10(3).

Shear stress-mediated vascular growth. / Tails, D. A.; Schriver, S. D.; Unthank, J. L.; Prewitt, R. L.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Tails, DA, Schriver, SD, Unthank, JL & Prewitt, RL 1996, 'Shear stress-mediated vascular growth', FASEB Journal, vol. 10, no. 3.
Tails DA, Schriver SD, Unthank JL, Prewitt RL. Shear stress-mediated vascular growth. FASEB Journal. 1996;10(3).
Tails, D. A. ; Schriver, S. D. ; Unthank, J. L. ; Prewitt, R. L. / Shear stress-mediated vascular growth. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
@article{575106707f634deaaac3526d7b0dea74,
title = "Shear stress-mediated vascular growth",
abstract = "In order to characterize in vivo shear stress-mediated vascular growth, adjacent rat mesenteric ileal and second-order arterial branches were ligated to induce blood flow increases through the immediately adjacent ileal artery. At one, three, and seven days animals were sacrificed and the mesenteric vasculature was perfusion-fixed and paraffin-embedded. Arterial cross-sections were cut and stained with toluidine blue for morphological assessment, and immunocytochemistry (ICC) was performed for proliferating cell nuclear antigen to measure DNA replication. Results indicate that, while arterial pressures were not altered by the ligation, ileal artery blood flow and shear stress were increased approximately 200{\%} initially after ligation. In arteries with elevated flow, mean lumen diameter and wall area increased significantly compared to same animal control vessels: ileal artery at seven days (255 ± 12 vs. 287 ± 12 μm; 11394 ± 1137 vs. 18016 ± 2648 um2, respectively), and second-order artery at three (188 ± 8 vs. 215 ± 9 μm; 7263 ± 591 vs. 11073 ± 1254 μm2, respectively) and seven (201 ± 22 vs. 277 ± 23 μm; 5883 ± 676 vs. 12936 ± 1684 μm2, respectively) days. ICC data suggest an initial medial hypertrophy in the larger vessel followed by medial hyperplasia, while the smaller vessel initially exhibits medial hyperplasia with subsequent medial cellular hypertrophy These data suggest that flowmediated vascular growth occurs through a combination of cellular hypertrophy and hyperplasia and is dependent on the order of the mesenteric arterial branch.",
author = "Tails, {D. A.} and Schriver, {S. D.} and Unthank, {J. L.} and Prewitt, {R. L.}",
year = "1996",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Shear stress-mediated vascular growth

AU - Tails, D. A.

AU - Schriver, S. D.

AU - Unthank, J. L.

AU - Prewitt, R. L.

PY - 1996

Y1 - 1996

N2 - In order to characterize in vivo shear stress-mediated vascular growth, adjacent rat mesenteric ileal and second-order arterial branches were ligated to induce blood flow increases through the immediately adjacent ileal artery. At one, three, and seven days animals were sacrificed and the mesenteric vasculature was perfusion-fixed and paraffin-embedded. Arterial cross-sections were cut and stained with toluidine blue for morphological assessment, and immunocytochemistry (ICC) was performed for proliferating cell nuclear antigen to measure DNA replication. Results indicate that, while arterial pressures were not altered by the ligation, ileal artery blood flow and shear stress were increased approximately 200% initially after ligation. In arteries with elevated flow, mean lumen diameter and wall area increased significantly compared to same animal control vessels: ileal artery at seven days (255 ± 12 vs. 287 ± 12 μm; 11394 ± 1137 vs. 18016 ± 2648 um2, respectively), and second-order artery at three (188 ± 8 vs. 215 ± 9 μm; 7263 ± 591 vs. 11073 ± 1254 μm2, respectively) and seven (201 ± 22 vs. 277 ± 23 μm; 5883 ± 676 vs. 12936 ± 1684 μm2, respectively) days. ICC data suggest an initial medial hypertrophy in the larger vessel followed by medial hyperplasia, while the smaller vessel initially exhibits medial hyperplasia with subsequent medial cellular hypertrophy These data suggest that flowmediated vascular growth occurs through a combination of cellular hypertrophy and hyperplasia and is dependent on the order of the mesenteric arterial branch.

AB - In order to characterize in vivo shear stress-mediated vascular growth, adjacent rat mesenteric ileal and second-order arterial branches were ligated to induce blood flow increases through the immediately adjacent ileal artery. At one, three, and seven days animals were sacrificed and the mesenteric vasculature was perfusion-fixed and paraffin-embedded. Arterial cross-sections were cut and stained with toluidine blue for morphological assessment, and immunocytochemistry (ICC) was performed for proliferating cell nuclear antigen to measure DNA replication. Results indicate that, while arterial pressures were not altered by the ligation, ileal artery blood flow and shear stress were increased approximately 200% initially after ligation. In arteries with elevated flow, mean lumen diameter and wall area increased significantly compared to same animal control vessels: ileal artery at seven days (255 ± 12 vs. 287 ± 12 μm; 11394 ± 1137 vs. 18016 ± 2648 um2, respectively), and second-order artery at three (188 ± 8 vs. 215 ± 9 μm; 7263 ± 591 vs. 11073 ± 1254 μm2, respectively) and seven (201 ± 22 vs. 277 ± 23 μm; 5883 ± 676 vs. 12936 ± 1684 μm2, respectively) days. ICC data suggest an initial medial hypertrophy in the larger vessel followed by medial hyperplasia, while the smaller vessel initially exhibits medial hyperplasia with subsequent medial cellular hypertrophy These data suggest that flowmediated vascular growth occurs through a combination of cellular hypertrophy and hyperplasia and is dependent on the order of the mesenteric arterial branch.

UR - http://www.scopus.com/inward/record.url?scp=33748965762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748965762&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -