Short- and Long-Term Effects of Growth Hormone (GH) Replacement on Protein Metabolism in GH-Deficient Adults

Jianjian Shi, Rajagopal V. Sekhar, Ashok Balasubramanyam, Kenneth Ellis, Peter J. Reeds, Farook Jahoor, Morali D. Sharma

Research output: Contribution to journalArticle

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Abstract

Reduced fat-free mass (FFM) in GH-deficient (GHD) adults is improved by GH replacement, but the protein metabolic changes are unclear. Using iv [ 2H3]leucine and oral 1-[13C1] leucine infusions and dual emission x-ray absorptiometry, we compared leucine kinetics and body composition in eight GHD adults and eight healthy controls in the fasted and fed states, before and after 2 wk and 6 months of GH replacement. Leucine kinetics were not different between pretreatment GHD subjects and controls. After 2 wk of GH treatment, leucine oxidation decreased in the GHD subjects compared with baseline values [fasted, 41 ± 6 vs. 30 ± 5 μmol/kg FFM·h (P <0.01); fed, 49 ± 3 vs. 41 ± 3.6 μmol/kg FFM·h (P <0.05)], leucine balance improved [fasted, -14 ± 4 vs. -3.5 ± 3 μmol/kg FFM·h (P <0.01); fed, 65 ± 10 vs. 72 ± 7 μmol/kg FFM·h (P = 0.07)], and protein synthesis increased [fasted, 116 ± 5 vs. 131 ± 6 μmol/kg FFM·h (P <0.05); fed, 103 ± 6 vs. 116 ± 6 μmol/kg FFM·h (P <0.05)]. After 6 months of GH treatment, these changes were not maintained in the fed state. The five GHD subjects with decreased FFM at baseline showed a significant increase after 6 months of GH treatment (P <0.05). GH replacement in GHD acutely improves protein balance by stimulating synthesis and inhibiting catabolism. After 6 months, protein kinetics reached a new homeostasis to maintain the net gain in FFM.

Original languageEnglish (US)
Pages (from-to)5827-5833
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume88
Issue number12
DOIs
StatePublished - Dec 2003
Externally publishedYes

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Metabolism
Growth Hormone
Leucine
Proteins
Fats
Kinetics
Body Composition
Homeostasis
X-Rays
X rays
Oxidation
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Short- and Long-Term Effects of Growth Hormone (GH) Replacement on Protein Metabolism in GH-Deficient Adults. / Shi, Jianjian; Sekhar, Rajagopal V.; Balasubramanyam, Ashok; Ellis, Kenneth; Reeds, Peter J.; Jahoor, Farook; Sharma, Morali D.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 88, No. 12, 12.2003, p. 5827-5833.

Research output: Contribution to journalArticle

Shi, Jianjian ; Sekhar, Rajagopal V. ; Balasubramanyam, Ashok ; Ellis, Kenneth ; Reeds, Peter J. ; Jahoor, Farook ; Sharma, Morali D. / Short- and Long-Term Effects of Growth Hormone (GH) Replacement on Protein Metabolism in GH-Deficient Adults. In: Journal of Clinical Endocrinology and Metabolism. 2003 ; Vol. 88, No. 12. pp. 5827-5833.
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