Short-chain fatty acids inhibit cAMP-mediated chloride secretion in rat colon

Pierre Dagher, Richard W. Egnor, Angelagrace Taglietta-Kohlbrecher, Alan N. Charney

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Butyrate stimulates salt absorption in mammalian colon. We examined whether butyrate also affects Cl- secretion. Mucosal segments of distal colon of male Sprague-Dawley rats and T84 cells were studied in Ussing chambers. In control colon, 1 mM dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) increased short-circuit current (I(sc)) and serosal- to-mucosal Cl- flux (J(sm)/(Cl)) by 3.2 ± 0.8 and 2.9 ± 0.8 μeq · cm- 2 · h-1, respectively. Mucosal or serosal 25 mM butyrate prevented DBcAMP-induced increases in I(sc) and J(sm)/(Cl). Four and eight millimolar butyrate caused half-maximal inhibition of the increases in J(sm)/(Cl) and I(sc), respectively. Butyrate also inhibited basal J(sm)/(Cl) (by 2.0 ± 0.4 μeq · cm-2 · h-1) but not carbachol-mediated Cl- secretion. The relative inhibitory potency at 25 mM of other short-chain fatty acids (SCFA) paralleled their degree of cellular metabolism: butyrate > acetate propionate > isobutyrate. At 25 mM, all SCFA reduced mucosal intracellular pH (pH(i)) transiently by 0.1 pH unit. In intact T84 cells, 50 mM butyrate inhibited the DBcAMP-induced rise in I(sc) by 55%. In T84 cells with nystatin-permeabilized basolateral membranes, butyrate inhibited the increase in I(sc) by 82%. We conclude that butyrate inhibits basal and cAMP-mediated Cl- secretion by a mechanism independent of pH(i), possibly located at the apical membrane.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number6 40-6
StatePublished - Dec 1996
Externally publishedYes

Fingerprint

Volatile Fatty Acids
Butyrates
Rats
Chlorides
Colon
Bucladesine
Isobutyrates
Membranes
Nystatin
Propionates
Carbachol
Metabolism
Short circuit currents
Cyclic AMP
Sprague Dawley Rats
Acetates
Salts
Fluxes

Keywords

  • adenosine 3',5'-cyclic monophosphate
  • butyrate
  • forskolin
  • intracellular pH
  • nystatin
  • T84 cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Short-chain fatty acids inhibit cAMP-mediated chloride secretion in rat colon. / Dagher, Pierre; Egnor, Richard W.; Taglietta-Kohlbrecher, Angelagrace; Charney, Alan N.

In: American Journal of Physiology - Cell Physiology, Vol. 271, No. 6 40-6, 12.1996.

Research output: Contribution to journalArticle

Dagher, Pierre ; Egnor, Richard W. ; Taglietta-Kohlbrecher, Angelagrace ; Charney, Alan N. / Short-chain fatty acids inhibit cAMP-mediated chloride secretion in rat colon. In: American Journal of Physiology - Cell Physiology. 1996 ; Vol. 271, No. 6 40-6.
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