Short-chain fatty acids inhibit cAMP-mediated chloride secretion in rat colon

Pierre C. Dagher, Richard W. Egnor, Angelagrace Taglietta-Kohlbrecher, Alan N. Charney

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Butyrate stimulates salt absorption in mammalian colon. We examined whether butyrate also affects Cl- secretion. Mucosal segments of distal colon of male Sprague-Dawley rats and T84 cells were studied in Ussing chambers. In control colon, 1 mM dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) increased short-circuit current (I(sc)) and serosal- to-mucosal Cl- flux (J(sm)/(Cl)) by 3.2 ± 0.8 and 2.9 ± 0.8 μeq · cm- 2 · h-1, respectively. Mucosal or serosal 25 mM butyrate prevented DBcAMP-induced increases in I(sc) and J(sm)/(Cl). Four and eight millimolar butyrate caused half-maximal inhibition of the increases in J(sm)/(Cl) and I(sc), respectively. Butyrate also inhibited basal J(sm)/(Cl) (by 2.0 ± 0.4 μeq · cm-2 · h-1) but not carbachol-mediated Cl- secretion. The relative inhibitory potency at 25 mM of other short-chain fatty acids (SCFA) paralleled their degree of cellular metabolism: butyrate > acetate propionate > isobutyrate. At 25 mM, all SCFA reduced mucosal intracellular pH (pH(i)) transiently by 0.1 pH unit. In intact T84 cells, 50 mM butyrate inhibited the DBcAMP-induced rise in I(sc) by 55%. In T84 cells with nystatin-permeabilized basolateral membranes, butyrate inhibited the increase in I(sc) by 82%. We conclude that butyrate inhibits basal and cAMP-mediated Cl- secretion by a mechanism independent of pH(i), possibly located at the apical membrane.

Original languageEnglish (US)
Pages (from-to)C1853-C1860
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number6 40-6
StatePublished - Dec 1 1996
Externally publishedYes

Keywords

  • T84 cells
  • adenosine 3',5'-cyclic monophosphate
  • butyrate
  • forskolin
  • intracellular pH
  • nystatin

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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  • Cite this

    Dagher, P. C., Egnor, R. W., Taglietta-Kohlbrecher, A., & Charney, A. N. (1996). Short-chain fatty acids inhibit cAMP-mediated chloride secretion in rat colon. American Journal of Physiology - Cell Physiology, 271(6 40-6), C1853-C1860.