SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis

Namit Sharma, Stephanie Everingham, Baskar Ramdas, Reuben Kapur, Andrew W B Craig

Research output: Contribution to journalArticle

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Abstract

SHP2 protein-tyrosine phosphatase (encoded by Ptpn11) positively regulates KIT (CD117) signaling in mast cells and is required for mast cell survival and homeostasis in mice. In this study, we uncover a role of SHP2 in promoting chemotaxis of mast cells toward stem cell factor (SCF), the ligand for KIT receptor. Using an inducible SHP2 knockout (KO) bone marrow-derived mast cell (BMMC) model, we observed defects in SCF-induced cell spreading, polarization, and chemotaxis. To address the mechanisms involved, we tested whether SHP2 promotes activation of Lyn kinase that was previously shown to promote mast cell chemotaxis. In SHP2 KO BMMCs, SCF-induced phosphorylation of the inhibitory C-terminal residue (pY507) was elevated compared with control cells, and phosphorylation of activation loop (pY396) was diminished. Because Lyn also was detected by substrate trapping assays, these results are consistent with SHP2 activating Lyn directly by dephosphorylation of pY507. Further analyses revealed a SHP2- and Lyn-dependent pathway leading to phosphorylation of Vav1, Rac activation, and F-actin polymerization in SCFtreated BMMCs. Treatment of BMMCs with a SHP2 inhibitor also led to impaired chemotaxis, consistent with SHP2 promoting SCF-induced chemotaxis of mast cells via a phosphatase-dependent mechanism. Thus, SHP2 inhibitors may be useful to limit SCF/KIT-induced mast cell recruitment to inflamed tissues or the tumor microenvironment.

Original languageEnglish
Pages (from-to)4859-4866
Number of pages8
JournalJournal of Immunology
Volume192
Issue number10
DOIs
StatePublished - May 15 2014

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Non-Receptor Type 11 Protein Tyrosine Phosphatase
Stem Cell Factor
Chemotaxis
Mast Cells
Phosphorylation
Protein Tyrosine Phosphatases
Tumor Microenvironment
Phosphoric Monoester Hydrolases
Polymerization
Actins
Cell Survival
Homeostasis
Phosphotransferases
Bone Marrow
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis. / Sharma, Namit; Everingham, Stephanie; Ramdas, Baskar; Kapur, Reuben; Craig, Andrew W B.

In: Journal of Immunology, Vol. 192, No. 10, 15.05.2014, p. 4859-4866.

Research output: Contribution to journalArticle

Sharma, Namit ; Everingham, Stephanie ; Ramdas, Baskar ; Kapur, Reuben ; Craig, Andrew W B. / SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis. In: Journal of Immunology. 2014 ; Vol. 192, No. 10. pp. 4859-4866.
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