Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus

Andrea DeDent, Taeok Bae, Dominique M. Missiakas, Olaf Schneewind

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Surface proteins of Gram-positive bacteria are covalently linked to the cell wall envelope by a mechanism requiring an N-terminal signal peptide and a C-terminal LPXTG motif sorting signal. We show here that surface proteins of Staphylococcus aureus arrive at two distinct destinations in the bacterial envelope, either distributed as a ring surrounding each cell or as discrete assembly sites. Proteins with ring-like distribution (clumping factor A (ClfA), Spa, fibronectin-binding protein B (FnbpB), serine-aspartate repeat protein C (SdrC) and SdrD) harbour signal peptides with a YSIRK/GS motif, whereas proteins directed to discrete assembly sites (S. aureus surface protein A (SasA), SasD, SasF and SasK) do not. Reciprocal exchange of signal peptides between surface proteins with (ClfA) or without the YSIRK/GS motif (SasF) directed recombinant products to the alternate destination, whereas mutations that altered only the YSIRK sequence had no effect. Our observations suggest that S. aureus distinguishes between signal peptides to address proteins to either the cell pole (signal peptides without YSIRK/GS) or the cross wall, the peptidoglycan layer that forms during cell division to separate new daughter cells (signal peptides with YISRK/GS motif).

Original languageEnglish
Pages (from-to)2656-2668
Number of pages13
JournalEMBO Journal
Volume27
Issue number20
DOIs
StatePublished - Oct 22 2008

Fingerprint

Protein Sorting Signals
Staphylococcus aureus
Membrane Proteins
Cells
Amino Acid Motifs
Proteins
Peptidoglycan
Staphylococcal Protein A
Gram-Positive Bacteria
Ports and harbors
Protein C
Sorting
Aspartic Acid
Cell Division
Cell Wall
Serine
Poles
Bacteria
Mutation

Keywords

  • Crosswall
  • Signal peptides
  • Staphylococcus aureus
  • Surface protein
  • YSIRK/GS motif

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus. / DeDent, Andrea; Bae, Taeok; Missiakas, Dominique M.; Schneewind, Olaf.

In: EMBO Journal, Vol. 27, No. 20, 22.10.2008, p. 2656-2668.

Research output: Contribution to journalArticle

DeDent, Andrea ; Bae, Taeok ; Missiakas, Dominique M. ; Schneewind, Olaf. / Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus. In: EMBO Journal. 2008 ; Vol. 27, No. 20. pp. 2656-2668.
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