Significant downregulation of platelet gene expression in metastatic lung cancer

David C. Calverley, Tzu L. Phang, Qamrul G. Choudhury, Bifeng Gao, Ana B. Oton, Michael J. Weyant, Mark W. Geraci

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Platelets play a major role in the metastatic dissemination of tumor cells in vivo. Recent evidence reveals megakaryocyte-derived platelet pre-mRNA is spliced to mRNA and then translated into functional proteins in response to external stimulation. Employing a human lung cancer model, we hypothesized a subset of megakaryocyte/platelet genes exists that are significantly over or underexpressed in metastasis compared with noncancer. Microarray analysis employing platelet mRNA followed by unsupervised hierarchical clustering revealed an expression profile that includes decreased expression of 197 of the 200 platelet genes with the most altered expression (p <1.0 × 10-4). Among the 608 splicing events identified between the metastasis and negative control groups, 33 highly variable genes were identified with between 3 and 13 splicing events each. In conclusion, this preliminary study reveals a platelet-based gene expression signature that differentiates metastatic lung cancer from negative controls on the basis of decreased expression of 197 of the 200 genes with the most altered expression levels. Further study may yield a prognostic tool for future metastasis among subsets of early stage lung cancer patients.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalClinical and Translational Science
Volume3
Issue number5
DOIs
StatePublished - Oct 2010
Externally publishedYes

Fingerprint

Platelets
Gene expression
Lung Neoplasms
Blood Platelets
Down-Regulation
Gene Expression
Genes
Megakaryocytes
Neoplasm Metastasis
Messenger RNA
RNA Precursors
Microarray Analysis
Microarrays
Transcriptome
Cluster Analysis
Tumors
Cells
Control Groups
Neoplasms
Proteins

Keywords

  • Lung cancer
  • Metastasis
  • Platelets

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Calverley, D. C., Phang, T. L., Choudhury, Q. G., Gao, B., Oton, A. B., Weyant, M. J., & Geraci, M. W. (2010). Significant downregulation of platelet gene expression in metastatic lung cancer. Clinical and Translational Science, 3(5), 227-232. https://doi.org/10.1111/j.1752-8062.2010.00226.x

Significant downregulation of platelet gene expression in metastatic lung cancer. / Calverley, David C.; Phang, Tzu L.; Choudhury, Qamrul G.; Gao, Bifeng; Oton, Ana B.; Weyant, Michael J.; Geraci, Mark W.

In: Clinical and Translational Science, Vol. 3, No. 5, 10.2010, p. 227-232.

Research output: Contribution to journalArticle

Calverley, DC, Phang, TL, Choudhury, QG, Gao, B, Oton, AB, Weyant, MJ & Geraci, MW 2010, 'Significant downregulation of platelet gene expression in metastatic lung cancer', Clinical and Translational Science, vol. 3, no. 5, pp. 227-232. https://doi.org/10.1111/j.1752-8062.2010.00226.x
Calverley, David C. ; Phang, Tzu L. ; Choudhury, Qamrul G. ; Gao, Bifeng ; Oton, Ana B. ; Weyant, Michael J. ; Geraci, Mark W. / Significant downregulation of platelet gene expression in metastatic lung cancer. In: Clinical and Translational Science. 2010 ; Vol. 3, No. 5. pp. 227-232.
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