Silencing by nuclear matrix attachment distinguishes cell-type specificity

Association with increased proliferation capacity

Amelia Linnemann, Stephen A. Krawetz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

DNA loop organization by nuclear scaffold/matrix attachment is a key regulator of gene expression that may provide a means to modulate phenotype. We have previously shown that attachment of genes to the NaCl-isolated nuclear matrix correlates with their silencing in HeLa cells. In contrast, expressed genes were associated with the lithium 3,5-diiodosalicylate (LIS)-isolated nuclear scaffold. To define their role in determining phenotype matrix attached regions (MARs) on human chromosomes 14-18 were identified as a function of expression in a primary cell line. The locations of MARs in aortic adventitial fibroblast (AoAF) cells were very stable (r = 0.909) and 96% of genes attached at MARs are silent (P < 0.001). Approximately one-third of the genes uniquely expressed in AoAF cells were associated with the HeLa cell nuclear matrix and silenced. Comparatively, 81% were associated with the AoAF cell nuclear scaffold (P < 0.001) and expressed. This suggests that nuclear scaffold/matrix association mediates a portion of cell type-specific gene expression thereby modulating phenotype. Interestingly, nuclear matrix attachment and thus silencing of specific genes that regulate proliferation and maintain the integrity of the HeLa cell genome suggests that transformation may at least in part be achieved through aberrant nuclear matrix attachment.

Original languageEnglish (US)
Pages (from-to)2779-2788
Number of pages10
JournalNucleic Acids Research
Volume37
Issue number9
DOIs
StatePublished - Jun 8 2009
Externally publishedYes

Fingerprint

Nuclear Matrix
Matrix Attachment Regions
Adventitia
HeLa Cells
Fibroblasts
Phenotype
Genes
Chromosomes, Human, Pair 18
Gene Expression
Chromosomes, Human, Pair 14
Human Chromosomes
Gene Silencing
Regulator Genes
Lithium
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Silencing by nuclear matrix attachment distinguishes cell-type specificity : Association with increased proliferation capacity. / Linnemann, Amelia; Krawetz, Stephen A.

In: Nucleic Acids Research, Vol. 37, No. 9, 08.06.2009, p. 2779-2788.

Research output: Contribution to journalArticle

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