Silencing S1P1 receptors regulates collagen-V reactive lymphocyte-mediated immunobiology in the transplanted lung

M. Chiyo, T. Iwata, T. J. Webb, Michael Vasko, E. L. Thompson, K. M. Heidler, Oscar Cummings, S. Yoshida, T. Fujisawa, D. D. Brand, D. S. Wilkes

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Type V collagen (col[V])-reactive lymphocytes contribute to lung transplant rejection, but the mechanisms for emigration into the graft are unknown. Sphingosine-1-phosphate-1 receptors (S1P1R) are believed to be required for lymphocyte emigration in other studies, but their role in col(V)-reactive lymphocyte rejection responses is not known. Utilizing small interfering RNA (siRNA) to reduce S1P1R expression on col(V)-reactive lymphocytes, we examined the role of S1P1R in the rejection response. Quantitative polymerase chain reaction (PCR) revealed strong expression of S1P1R messenger RNA (mRNA) on col(V)-reactive lymphocytes isolated from immunized rats. S1P1R-specific siRNA (S1P1R siRNA) reduced expression of S1P1R mRNA and protein, whereas scramble siRNA (SC siRNA) had no effect. Adoptive transfer of lymphocytes treated with S1P1R siRNA to rat Wistar Kyoto (WKY) lung isograft recipients resulted in retention of cells within the liver with fewer cells in mediastinal lymph nodes when compared to cells exposed to SC siRNA. S1P1R-deficient cells proliferated in response to alloantigens, but not in response to col(V), and produced less interferon (IFN)-γ in response to col(V) compared to controls. Downregulating S1P1R did not affect production of interleukin (IL)-10 and tumor necrosis factor (TNF)-α, or expression of adhesion molecules critical for migration, but prevented rejection pathology and lowered local levels of IFN-γ post adoptive transfer. These data demonstrate novel roles of S1P1R, which include regulating emigration and modulating lymphocyte activation.

Original languageEnglish
Pages (from-to)537-546
Number of pages10
JournalAmerican Journal of Transplantation
Volume8
Issue number3
DOIs
StatePublished - Mar 2008

Fingerprint

Lysosphingolipid Receptors
Collagen
Lymphocytes
Lung
Small Interfering RNA
Emigration and Immigration
Adoptive Transfer
Interferons
Collagen Type V
Isografts
Messenger RNA
Isoantigens
Inbred WKY Rats
Graft Rejection
Lymphocyte Activation
Interleukin-10
Down-Regulation
Tumor Necrosis Factor-alpha
Lymph Nodes

Keywords

  • Autoimmunity
  • Cell trafficking
  • Lung
  • T cells
  • Transplantation

ASJC Scopus subject areas

  • Immunology

Cite this

Silencing S1P1 receptors regulates collagen-V reactive lymphocyte-mediated immunobiology in the transplanted lung. / Chiyo, M.; Iwata, T.; Webb, T. J.; Vasko, Michael; Thompson, E. L.; Heidler, K. M.; Cummings, Oscar; Yoshida, S.; Fujisawa, T.; Brand, D. D.; Wilkes, D. S.

In: American Journal of Transplantation, Vol. 8, No. 3, 03.2008, p. 537-546.

Research output: Contribution to journalArticle

Chiyo, M. ; Iwata, T. ; Webb, T. J. ; Vasko, Michael ; Thompson, E. L. ; Heidler, K. M. ; Cummings, Oscar ; Yoshida, S. ; Fujisawa, T. ; Brand, D. D. ; Wilkes, D. S. / Silencing S1P1 receptors regulates collagen-V reactive lymphocyte-mediated immunobiology in the transplanted lung. In: American Journal of Transplantation. 2008 ; Vol. 8, No. 3. pp. 537-546.
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