Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation

Qiang Fu, Mohamed N. Rahaman, B. Sonny Bal, Lynda Bonewald, Keiichi Kuroki, Roger F. Brown

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

In Part I, the in vitro degradation of bioactive glass scaffolds with a microstructure similar to that of human trabecular bone, but with three different compositions, was investigated as a function of immersion time in a simulated body fluid. The glasses consisted of a silicate (13-93) composition, a borosilicate composition (designated 13-93B1), and a borate composition (13-93B3), in which one-third or all of the SiO2 content of 13-93 was replaced by B2O3, respectively. This work is an extension of Part I, to investigate the effect of the glass composition on the in vitro response of osteogenic MLO-A5 cells to these scaffolds, and on the ability of the scaffolds to support tissue infiltration in a rat subcutaneous implantation model. The results of assays for cell viability and alkaline phosphatase activity showed that the slower degrading silicate 13-93 and borosilicate 13-93B1 scaffolds were far better than the borate 13-93B3 scaffolds in supporting cell proliferation and function. However, all three groups of scaffolds showed the ability to support tissue infiltration in vivo after implantation for 6 weeks. The results indicate that the required bioactivity and degradation rate may be achieved by substituting an appropriate amount of SiO2 in 13-93 glass with B2O3, and that these trabecular glass scaffolds could serve as substrates for the repair and regeneration of contained bone defects.

Original languageEnglish (US)
Pages (from-to)172-179
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume95
Issue number1
DOIs
StatePublished - Oct 2010
Externally publishedYes

Fingerprint

Silicates
Bioactive glass
Borates
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Glass
Bone
Bone and Bones
Degradation
Chemical analysis
Infiltration
Bone Regeneration
Tissue
Body Fluids
Immersion
Body fluids
Phosphatases
Cell proliferation

Keywords

  • Bioactive glass
  • Bone repair
  • Cell culture
  • Scaffold
  • Subcutaneous implantation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

Cite this

Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation. / Fu, Qiang; Rahaman, Mohamed N.; Bal, B. Sonny; Bonewald, Lynda; Kuroki, Keiichi; Brown, Roger F.

In: Journal of Biomedical Materials Research - Part A, Vol. 95, No. 1, 10.2010, p. 172-179.

Research output: Contribution to journalArticle

@article{14893db7c8a1428698990a00d121fbe5,
title = "Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation",
abstract = "In Part I, the in vitro degradation of bioactive glass scaffolds with a microstructure similar to that of human trabecular bone, but with three different compositions, was investigated as a function of immersion time in a simulated body fluid. The glasses consisted of a silicate (13-93) composition, a borosilicate composition (designated 13-93B1), and a borate composition (13-93B3), in which one-third or all of the SiO2 content of 13-93 was replaced by B2O3, respectively. This work is an extension of Part I, to investigate the effect of the glass composition on the in vitro response of osteogenic MLO-A5 cells to these scaffolds, and on the ability of the scaffolds to support tissue infiltration in a rat subcutaneous implantation model. The results of assays for cell viability and alkaline phosphatase activity showed that the slower degrading silicate 13-93 and borosilicate 13-93B1 scaffolds were far better than the borate 13-93B3 scaffolds in supporting cell proliferation and function. However, all three groups of scaffolds showed the ability to support tissue infiltration in vivo after implantation for 6 weeks. The results indicate that the required bioactivity and degradation rate may be achieved by substituting an appropriate amount of SiO2 in 13-93 glass with B2O3, and that these trabecular glass scaffolds could serve as substrates for the repair and regeneration of contained bone defects.",
keywords = "Bioactive glass, Bone repair, Cell culture, Scaffold, Subcutaneous implantation",
author = "Qiang Fu and Rahaman, {Mohamed N.} and Bal, {B. Sonny} and Lynda Bonewald and Keiichi Kuroki and Brown, {Roger F.}",
year = "2010",
month = "10",
doi = "10.1002/jbm.a.32823",
language = "English (US)",
volume = "95",
pages = "172--179",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation

AU - Fu, Qiang

AU - Rahaman, Mohamed N.

AU - Bal, B. Sonny

AU - Bonewald, Lynda

AU - Kuroki, Keiichi

AU - Brown, Roger F.

PY - 2010/10

Y1 - 2010/10

N2 - In Part I, the in vitro degradation of bioactive glass scaffolds with a microstructure similar to that of human trabecular bone, but with three different compositions, was investigated as a function of immersion time in a simulated body fluid. The glasses consisted of a silicate (13-93) composition, a borosilicate composition (designated 13-93B1), and a borate composition (13-93B3), in which one-third or all of the SiO2 content of 13-93 was replaced by B2O3, respectively. This work is an extension of Part I, to investigate the effect of the glass composition on the in vitro response of osteogenic MLO-A5 cells to these scaffolds, and on the ability of the scaffolds to support tissue infiltration in a rat subcutaneous implantation model. The results of assays for cell viability and alkaline phosphatase activity showed that the slower degrading silicate 13-93 and borosilicate 13-93B1 scaffolds were far better than the borate 13-93B3 scaffolds in supporting cell proliferation and function. However, all three groups of scaffolds showed the ability to support tissue infiltration in vivo after implantation for 6 weeks. The results indicate that the required bioactivity and degradation rate may be achieved by substituting an appropriate amount of SiO2 in 13-93 glass with B2O3, and that these trabecular glass scaffolds could serve as substrates for the repair and regeneration of contained bone defects.

AB - In Part I, the in vitro degradation of bioactive glass scaffolds with a microstructure similar to that of human trabecular bone, but with three different compositions, was investigated as a function of immersion time in a simulated body fluid. The glasses consisted of a silicate (13-93) composition, a borosilicate composition (designated 13-93B1), and a borate composition (13-93B3), in which one-third or all of the SiO2 content of 13-93 was replaced by B2O3, respectively. This work is an extension of Part I, to investigate the effect of the glass composition on the in vitro response of osteogenic MLO-A5 cells to these scaffolds, and on the ability of the scaffolds to support tissue infiltration in a rat subcutaneous implantation model. The results of assays for cell viability and alkaline phosphatase activity showed that the slower degrading silicate 13-93 and borosilicate 13-93B1 scaffolds were far better than the borate 13-93B3 scaffolds in supporting cell proliferation and function. However, all three groups of scaffolds showed the ability to support tissue infiltration in vivo after implantation for 6 weeks. The results indicate that the required bioactivity and degradation rate may be achieved by substituting an appropriate amount of SiO2 in 13-93 glass with B2O3, and that these trabecular glass scaffolds could serve as substrates for the repair and regeneration of contained bone defects.

KW - Bioactive glass

KW - Bone repair

KW - Cell culture

KW - Scaffold

KW - Subcutaneous implantation

UR - http://www.scopus.com/inward/record.url?scp=77956468013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956468013&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.32823

DO - 10.1002/jbm.a.32823

M3 - Article

C2 - 20540099

AN - SCOPUS:77956468013

VL - 95

SP - 172

EP - 179

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 1

ER -