Silver staining (Campbell-Switzer) of neuronal α-synuclein assemblies induced by multiple system atrophy and Parkinson's disease brain extracts in transgenic mice

Isabelle Lavenir, Daniela Passarella, Masami Masuda-Suzukake, Annabelle Curry, Janice L. Holton, Bernardino Ghetti, Michel Goedert

Research output: Contribution to journalArticle

Abstract

Synucleinopathies [Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA)] share filamentous α-synuclein assemblies in nerve cells and glial cells. We compared the abilities of brain extracts from MSA and PD patients to induce neuronal α-synuclein assembly and neurodegeneration following intracerebral injection in heterozygous mice transgenic for human mutant A53T α-synuclein. MSA extracts were more potent than PD extracts in inducing α-synuclein assembly and in causing neurodegeneration. MSA assemblies were Campbell-Switzer- and Gallyas-silver-positive, whereas PD assemblies were only Campbell-Switzer-positive, in confirmation of previous findings. However, induced α-synuclein inclusions were invariably Campbell-Switzer-positive and Gallyas-negative, irrespective of whether MSA or PD brain extracts were injected. The α-synuclein inclusions of non-injected homozygous mice transgenic for A53T α-synuclein were also Campbell-Switzer-positive and Gallyas-negative. These findings demonstrate that transgene expression and its intracellular environment dominated over the silver staining properties of the conformers of assembled α-synuclein.

Original languageEnglish (US)
Number of pages1
JournalActa neuropathologica communications
Volume7
Issue number1
DOIs
StatePublished - Sep 16 2019

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Synucleins
Multiple System Atrophy
Silver Staining
Transgenic Mice
Parkinson Disease
Brain
Lewy Body Disease
Aptitude
Transgenes
Silver
Neuroglia
Neurons

Keywords

  • Multiple system atrophy
  • Parkinson’s disease
  • Seeding
  • Silver staining
  • Transgenic
  • α-Synuclein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Silver staining (Campbell-Switzer) of neuronal α-synuclein assemblies induced by multiple system atrophy and Parkinson's disease brain extracts in transgenic mice. / Lavenir, Isabelle; Passarella, Daniela; Masuda-Suzukake, Masami; Curry, Annabelle; Holton, Janice L.; Ghetti, Bernardino; Goedert, Michel.

In: Acta neuropathologica communications, Vol. 7, No. 1, 16.09.2019.

Research output: Contribution to journalArticle

Lavenir, Isabelle ; Passarella, Daniela ; Masuda-Suzukake, Masami ; Curry, Annabelle ; Holton, Janice L. ; Ghetti, Bernardino ; Goedert, Michel. / Silver staining (Campbell-Switzer) of neuronal α-synuclein assemblies induced by multiple system atrophy and Parkinson's disease brain extracts in transgenic mice. In: Acta neuropathologica communications. 2019 ; Vol. 7, No. 1.
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