Similar repopulating capacity of mitotically active and resting umbilical cord similar blood CD34 + cells in NOD/SCID mice

J. Wilpshaar, J. H F Falkenburg, X. Tong, W. A. Noort, R. Breese, D. Heilman, H. Kanhai, Christie Orschell, Edward Srour

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Abstract

It was hypothesized that during mammalian development, the extensive need for hematopoietic cells requires equal contribution to blood cell production from both quiescent and cycling hematopoietic stem cells (HSCs) while maintaining the stem cell pool. To investigate this hypothesis, the engraftment potential of umbilical cord blood (UCB) CD34+ cells residing in either G0 (G0CD34+ cells) or G1 (G1CD34+ cells) phases of the cell cycle was assessed in nonobese diabetic/severe combined immune-deficient (NOD/SCID) mice. Whereas the level of chimerism in mice transplanted with UCB G0CD34+ cells was 69.9% ± 24.0%, mice receiving equal numbers of G1CD34+ cells harbored 46.7% ± 21.3% human cells 8 weeks posttransplantation. Both groups of cells sustained multilineage differentiation and the production of CD34+ cells in recipient animals. The relationship between the number of transplanted G0CD34+ or G1CD34+ cells and the level of chimerism was analyzed by a general linear models procedure. Although the initial level of chimerism following transplantation of G0CD34+ cells was higher than that sustained by G1CD34+ cells, the increment in the degree of chimerism obtained with each additional 103 cells of either phenotype was identical, suggesting that the reconstitution potential of these 2 types of cells was similar. Of interest is that human cells recovered from primary recipients of both G0CD34+ and G1CD34+ cells engrafted in secondary NOD/SCID recipients, albeit at a substantially lower level, confirming the primitive nature of UCB CD34+ cells residing in G1. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)2100-2107
Number of pages8
JournalBlood
Volume96
Issue number6
StatePublished - Sep 15 2000

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Fetal Blood
Blood Cells
Blood
Cells
Stem cells
Chimerism
Animals
Cell Transplantation
Hematopoietic Stem Cells
Linear Models
Cell Cycle
Stem Cells
Cell Count
Phenotype

ASJC Scopus subject areas

  • Hematology

Cite this

Wilpshaar, J., Falkenburg, J. H. F., Tong, X., Noort, W. A., Breese, R., Heilman, D., ... Srour, E. (2000). Similar repopulating capacity of mitotically active and resting umbilical cord similar blood CD34 + cells in NOD/SCID mice. Blood, 96(6), 2100-2107.

Similar repopulating capacity of mitotically active and resting umbilical cord similar blood CD34 + cells in NOD/SCID mice. / Wilpshaar, J.; Falkenburg, J. H F; Tong, X.; Noort, W. A.; Breese, R.; Heilman, D.; Kanhai, H.; Orschell, Christie; Srour, Edward.

In: Blood, Vol. 96, No. 6, 15.09.2000, p. 2100-2107.

Research output: Contribution to journalArticle

Wilpshaar, J, Falkenburg, JHF, Tong, X, Noort, WA, Breese, R, Heilman, D, Kanhai, H, Orschell, C & Srour, E 2000, 'Similar repopulating capacity of mitotically active and resting umbilical cord similar blood CD34 + cells in NOD/SCID mice', Blood, vol. 96, no. 6, pp. 2100-2107.
Wilpshaar J, Falkenburg JHF, Tong X, Noort WA, Breese R, Heilman D et al. Similar repopulating capacity of mitotically active and resting umbilical cord similar blood CD34 + cells in NOD/SCID mice. Blood. 2000 Sep 15;96(6):2100-2107.
Wilpshaar, J. ; Falkenburg, J. H F ; Tong, X. ; Noort, W. A. ; Breese, R. ; Heilman, D. ; Kanhai, H. ; Orschell, Christie ; Srour, Edward. / Similar repopulating capacity of mitotically active and resting umbilical cord similar blood CD34 + cells in NOD/SCID mice. In: Blood. 2000 ; Vol. 96, No. 6. pp. 2100-2107.
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