Similarity of vasorelaxant effects of natriuretic peptides in isolated blood vessels of salmonids

M. P. Smith, Y. Takei, Kenneth Olson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Natriuretic peptides (NPs) have been implicated in cardiovascular regulation in rainbow trout (Oncorhyncus mykiss), and it has been observed that the vasorelaxant activity of distinct trout and human NPs is similar in isolated trout arteries. This study characterizes the response of a variety of vessels from rainbow trout and other salmonids to different NPs. The effects of heterologous (rat atrial and human atrial) and homologous (rainbow trout atrial and rainbow trout ventricular) NPs were examined in precontracted efferent branchial arteries from rainbow trout (O. mykiss, Kamloops strain), lake whitefish (Coregonus clupeaformis), and in rainbow trout celiacomesenteric arteries and anterior cardinal veins. The response to mammalian NPs was also examined in efferent branchial arteries from the steelhead (O. mykiss, Skamania strain), coho salmon (Oncorhyncus kisutch), brook trout (Salvelinus fontinalis), and brown trout (Salmo trutta). In general, there were relatively few differences that were species, peptide, or vessel specific. There was no difference in the sensitivity (concentration producing a half-maximal response EC50) or efficacy (percent relaxation, i.e., E(max)) of trout or whitefish efferent branchial arteries to any NP, except human NP, which was significantly less effective (greater EC50 and lower E(max)) in whitefish arteries. There were no differences in E(max) of mammalian NPs in efferent branchial arteries from any species, and only coho and brook trout had significantly different EC50's (coho, 1.0 ± 0.2 nM; brook trout, 4.2 ± 0.6 nM; and other species, from 1.9 to 3.5 nM). Rainbow and coho anterior cardinal veins were less sensitive than arteries to mammalian NPs (EC50's; 8.8 ± 2.0, 2.0 ± 0.1 vs. 3.0 ± 0.9, 1.0 ± 0.2, respectively), whereas brown trout veins were more sensitive (1.0 ± 0.2, 3.5 ± 1.3, respectively). Sodium nitroprusside (SNP), which activates soluble guanylate cyclase, was vasodilatory, albeit significantly less potent than all NPs, in efferent branchial arteries of all species. SNP was significantly more potent in trout than whitefish efferent branchial arteries, whereas it was equally efficacious in these vessels. These results demonstrate that multiple vessels from various salmonids are similarly responsive to the vasorelaxant effects of a variety of NPs and that the salmonid NP receptor has relatively little ability to discriminate between homologous and heterotogous peptides. We conclude that the vascular NP receptor complex is highly conserved among salmonids. Further, salmonids utilize cyclic guanosine monophosphate (cGMP) elevations for reductions of vascular tonus by both particulate and soluble guanylate cyclase pathways.

Original languageEnglish
Pages (from-to)494-500
Number of pages7
JournalPhysiological and Biochemical Zoology
Volume73
Issue number4
DOIs
StatePublished - 2000

Fingerprint

Salmonidae
natriuretic peptides
Natriuretic Peptides
vasodilation
Blood vessels
Vasodilator Agents
blood vessels
Trout
Blood Vessels
arteries
Oncorhynchus mykiss
Arteries
Salvelinus fontinalis
trout
Salmo trutta
Coregonus clupeaformis
guanylate cyclase
Oncorhynchus kisutch
Veins
Peptide Receptors

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Physiology
  • Physiology (medical)

Cite this

Similarity of vasorelaxant effects of natriuretic peptides in isolated blood vessels of salmonids. / Smith, M. P.; Takei, Y.; Olson, Kenneth.

In: Physiological and Biochemical Zoology, Vol. 73, No. 4, 2000, p. 494-500.

Research output: Contribution to journalArticle

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N2 - Natriuretic peptides (NPs) have been implicated in cardiovascular regulation in rainbow trout (Oncorhyncus mykiss), and it has been observed that the vasorelaxant activity of distinct trout and human NPs is similar in isolated trout arteries. This study characterizes the response of a variety of vessels from rainbow trout and other salmonids to different NPs. The effects of heterologous (rat atrial and human atrial) and homologous (rainbow trout atrial and rainbow trout ventricular) NPs were examined in precontracted efferent branchial arteries from rainbow trout (O. mykiss, Kamloops strain), lake whitefish (Coregonus clupeaformis), and in rainbow trout celiacomesenteric arteries and anterior cardinal veins. The response to mammalian NPs was also examined in efferent branchial arteries from the steelhead (O. mykiss, Skamania strain), coho salmon (Oncorhyncus kisutch), brook trout (Salvelinus fontinalis), and brown trout (Salmo trutta). In general, there were relatively few differences that were species, peptide, or vessel specific. There was no difference in the sensitivity (concentration producing a half-maximal response EC50) or efficacy (percent relaxation, i.e., E(max)) of trout or whitefish efferent branchial arteries to any NP, except human NP, which was significantly less effective (greater EC50 and lower E(max)) in whitefish arteries. There were no differences in E(max) of mammalian NPs in efferent branchial arteries from any species, and only coho and brook trout had significantly different EC50's (coho, 1.0 ± 0.2 nM; brook trout, 4.2 ± 0.6 nM; and other species, from 1.9 to 3.5 nM). Rainbow and coho anterior cardinal veins were less sensitive than arteries to mammalian NPs (EC50's; 8.8 ± 2.0, 2.0 ± 0.1 vs. 3.0 ± 0.9, 1.0 ± 0.2, respectively), whereas brown trout veins were more sensitive (1.0 ± 0.2, 3.5 ± 1.3, respectively). Sodium nitroprusside (SNP), which activates soluble guanylate cyclase, was vasodilatory, albeit significantly less potent than all NPs, in efferent branchial arteries of all species. SNP was significantly more potent in trout than whitefish efferent branchial arteries, whereas it was equally efficacious in these vessels. These results demonstrate that multiple vessels from various salmonids are similarly responsive to the vasorelaxant effects of a variety of NPs and that the salmonid NP receptor has relatively little ability to discriminate between homologous and heterotogous peptides. We conclude that the vascular NP receptor complex is highly conserved among salmonids. Further, salmonids utilize cyclic guanosine monophosphate (cGMP) elevations for reductions of vascular tonus by both particulate and soluble guanylate cyclase pathways.

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