Simultaneous processing of substrate-dependent monolayer cultures for scanning and transmission electron microscopy

G. S. Dougherty, J. A. McAteer, Andrew Evan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A reliable method is described for processing substrate-dependent cells raised on culture-grade plastic for both scanning (SEM) and transmission electron microscopy (TEM). This technique allows collection of specimens for TEM and SEM from the same culture dish or flask. In this way it is possible to study the surface morphology (SEM) and thin section ultrastructure of cells from contiguous regions of a culture. Specific regions of a culture can be selected and processed so that specimens retain orientation throughout mounting or embedment and sectioning. The method is applicable both to confluent cultures as well as isolated colonies.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalJournal of Tissue Culture Methods
Volume10
Issue number4
DOIs
StatePublished - Dec 1986

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Scanning Transmission Electron Microscopy
Transmission Electron Microscopy
Surface morphology
Monolayers
Transmission electron microscopy
Specimen Handling
Scanning electron microscopy
Substrates
Processing
Plastics
Mountings
Scanning

Keywords

  • monolayer culture
  • scanning electron microscopy
  • transmission electron microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Simultaneous processing of substrate-dependent monolayer cultures for scanning and transmission electron microscopy. / Dougherty, G. S.; McAteer, J. A.; Evan, Andrew.

In: Journal of Tissue Culture Methods, Vol. 10, No. 4, 12.1986, p. 239-244.

Research output: Contribution to journalArticle

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