Simultaneous processing of substrate-dependent monolayer cultures for scanning and transmission electron microscopy

G. S. Dougherty, J. A. McAteer, A. P. Evan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A reliable method is described for processing substrate-dependent cells raised on culture-grade plastic for both scanning (SEM) and transmission electron microscopy (TEM). This technique allows collection of specimens for TEM and SEM from the same culture dish or flask. In this way it is possible to study the surface morphology (SEM) and thin section ultrastructure of cells from contiguous regions of a culture. Specific regions of a culture can be selected and processed so that specimens retain orientation throughout mounting or embedment and sectioning. The method is applicable both to confluent cultures as well as isolated colonies.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalJournal of Tissue Culture Methods
Volume10
Issue number4
DOIs
StatePublished - Dec 1986

Keywords

  • monolayer culture
  • scanning electron microscopy
  • transmission electron microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Fingerprint Dive into the research topics of 'Simultaneous processing of substrate-dependent monolayer cultures for scanning and transmission electron microscopy'. Together they form a unique fingerprint.

  • Cite this