Simultaneous ribosome profiling of human host cells infected with Toxoplasma gondii

Michael J. Holmes, Premal Shah, Ronald Wek, William Sullivan

Research output: Contribution to journalArticle

Abstract

Toxoplasma gondii is a ubiquitous obligate intracellular parasite that infects the nucleated cells of warm-blooded animals. From within the parasitophorous vacuole in which they reside, Toxoplasma tachyzoites secrete an arsenal of effector proteins that can reprogram host gene expression to facilitate parasite survival and replication. Gaining a better understanding of how host gene expression is altered upon infection is central for understanding parasite strategies for host invasion and for developing new parasite therapies. Here, we applied ribosome profiling coupled with mRNA measurements to concurrently study gene expression in the parasite and in host human foreskin fibroblasts. By examining the parasite transcriptome and translatome, we identified potential upstream open reading frames that may permit the stress-induced preferential translation of parasite mRNAs. We also determined that tachyzoites reduce host death-associated pathways and increase survival, proliferation, and motility in both quiescent and proliferative host cell models of infection. Additionally, proliferative cells alter their gene expression in ways that are consistent with massive transcriptional rewiring, while quiescent cells were best characterized by reentry into the cell cycle. We also identified a translational control regimen consistent with mechanistic target of rapamycin (mTOR) activation in quiescent cells and, to a lesser degree, in proliferative cells. This study illustrates the utility of the method for dissection of gene expression programs simultaneously in the parasite and host.

Original languageEnglish (US)
Article numbere00292-19
JournalmSphere
Volume4
Issue number3
DOIs
StatePublished - May 1 2019

Fingerprint

Toxoplasma
Ribosomes
Parasites
Gene Expression
Foreskin
Protein Biosynthesis
Sirolimus
Vacuoles
Infection
Transcriptome
Open Reading Frames
Dissection
Cell Cycle
Fibroblasts
Messenger RNA
Survival

Keywords

  • Apicomplexa
  • Parasites
  • Ribosomal profiling
  • Toxoplasma
  • Translation
  • Translation control

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Simultaneous ribosome profiling of human host cells infected with Toxoplasma gondii. / Holmes, Michael J.; Shah, Premal; Wek, Ronald; Sullivan, William.

In: mSphere, Vol. 4, No. 3, e00292-19, 01.05.2019.

Research output: Contribution to journalArticle

@article{fde71f6eee9046a5a30e11b0b6be2ad8,
title = "Simultaneous ribosome profiling of human host cells infected with Toxoplasma gondii",
abstract = "Toxoplasma gondii is a ubiquitous obligate intracellular parasite that infects the nucleated cells of warm-blooded animals. From within the parasitophorous vacuole in which they reside, Toxoplasma tachyzoites secrete an arsenal of effector proteins that can reprogram host gene expression to facilitate parasite survival and replication. Gaining a better understanding of how host gene expression is altered upon infection is central for understanding parasite strategies for host invasion and for developing new parasite therapies. Here, we applied ribosome profiling coupled with mRNA measurements to concurrently study gene expression in the parasite and in host human foreskin fibroblasts. By examining the parasite transcriptome and translatome, we identified potential upstream open reading frames that may permit the stress-induced preferential translation of parasite mRNAs. We also determined that tachyzoites reduce host death-associated pathways and increase survival, proliferation, and motility in both quiescent and proliferative host cell models of infection. Additionally, proliferative cells alter their gene expression in ways that are consistent with massive transcriptional rewiring, while quiescent cells were best characterized by reentry into the cell cycle. We also identified a translational control regimen consistent with mechanistic target of rapamycin (mTOR) activation in quiescent cells and, to a lesser degree, in proliferative cells. This study illustrates the utility of the method for dissection of gene expression programs simultaneously in the parasite and host.",
keywords = "Apicomplexa, Parasites, Ribosomal profiling, Toxoplasma, Translation, Translation control",
author = "Holmes, {Michael J.} and Premal Shah and Ronald Wek and William Sullivan",
year = "2019",
month = "5",
day = "1",
doi = "10.1128/mSphere.00292-19",
language = "English (US)",
volume = "4",
journal = "mSphere",
issn = "2379-5042",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Simultaneous ribosome profiling of human host cells infected with Toxoplasma gondii

AU - Holmes, Michael J.

AU - Shah, Premal

AU - Wek, Ronald

AU - Sullivan, William

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Toxoplasma gondii is a ubiquitous obligate intracellular parasite that infects the nucleated cells of warm-blooded animals. From within the parasitophorous vacuole in which they reside, Toxoplasma tachyzoites secrete an arsenal of effector proteins that can reprogram host gene expression to facilitate parasite survival and replication. Gaining a better understanding of how host gene expression is altered upon infection is central for understanding parasite strategies for host invasion and for developing new parasite therapies. Here, we applied ribosome profiling coupled with mRNA measurements to concurrently study gene expression in the parasite and in host human foreskin fibroblasts. By examining the parasite transcriptome and translatome, we identified potential upstream open reading frames that may permit the stress-induced preferential translation of parasite mRNAs. We also determined that tachyzoites reduce host death-associated pathways and increase survival, proliferation, and motility in both quiescent and proliferative host cell models of infection. Additionally, proliferative cells alter their gene expression in ways that are consistent with massive transcriptional rewiring, while quiescent cells were best characterized by reentry into the cell cycle. We also identified a translational control regimen consistent with mechanistic target of rapamycin (mTOR) activation in quiescent cells and, to a lesser degree, in proliferative cells. This study illustrates the utility of the method for dissection of gene expression programs simultaneously in the parasite and host.

AB - Toxoplasma gondii is a ubiquitous obligate intracellular parasite that infects the nucleated cells of warm-blooded animals. From within the parasitophorous vacuole in which they reside, Toxoplasma tachyzoites secrete an arsenal of effector proteins that can reprogram host gene expression to facilitate parasite survival and replication. Gaining a better understanding of how host gene expression is altered upon infection is central for understanding parasite strategies for host invasion and for developing new parasite therapies. Here, we applied ribosome profiling coupled with mRNA measurements to concurrently study gene expression in the parasite and in host human foreskin fibroblasts. By examining the parasite transcriptome and translatome, we identified potential upstream open reading frames that may permit the stress-induced preferential translation of parasite mRNAs. We also determined that tachyzoites reduce host death-associated pathways and increase survival, proliferation, and motility in both quiescent and proliferative host cell models of infection. Additionally, proliferative cells alter their gene expression in ways that are consistent with massive transcriptional rewiring, while quiescent cells were best characterized by reentry into the cell cycle. We also identified a translational control regimen consistent with mechanistic target of rapamycin (mTOR) activation in quiescent cells and, to a lesser degree, in proliferative cells. This study illustrates the utility of the method for dissection of gene expression programs simultaneously in the parasite and host.

KW - Apicomplexa

KW - Parasites

KW - Ribosomal profiling

KW - Toxoplasma

KW - Translation

KW - Translation control

UR - http://www.scopus.com/inward/record.url?scp=85067078584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067078584&partnerID=8YFLogxK

U2 - 10.1128/mSphere.00292-19

DO - 10.1128/mSphere.00292-19

M3 - Article

C2 - 31167946

AN - SCOPUS:85067078584

VL - 4

JO - mSphere

JF - mSphere

SN - 2379-5042

IS - 3

M1 - e00292-19

ER -