Single-Cell Lineage Tracing Reveals that Oriented Cell Division Contributes to Trabecular Morphogenesis and Regional Specification

Jingjing Li, Lianjie Miao, David Shieh, Ernest Spiotto, Jian Li, Bin Zhou, Antoni Paul, Robert J. Schwartz, Anthony B. Firulli, Harold A. Singer, Guoying Huang, Mingfu Wu

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22 Scopus citations

Abstract

The cardiac trabeculae are sheet-like structures extending from the myocardium that function to increase surface area. A lack of trabeculation causes embryonic lethality due to compromised cardiac function. To understand the cellular and molecular mechanisms of trabecular formation, we genetically labeled individual cardiomyocytes prior to trabeculation via the brainbow multicolor system and traced and analyzed the labeled cells during trabeculation by whole-embryo clearing and imaging. The clones derived from labeled single cells displayed four different geometric patterns that are derived from different patterns of oriented cell division (OCD) and migration. Of the four types of clones, the inner, transmural, and mixed clones contributed to trabecular cardiomyocytes. Further studies showed that perpendicular OCD is an extrinsic asymmetric cell division that putatively contributes to trabecular regional specification. Furthermore, N-Cadherin deletion in labeled clones disrupted the clonal patterns. In summary, our data demonstrate that OCD contributes to trabecular morphogenesis and specification.

Original languageEnglish (US)
Pages (from-to)158-170
Number of pages13
JournalCell Reports
Volume15
Issue number1
DOIs
StatePublished - Apr 5 2016

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Li, J., Miao, L., Shieh, D., Spiotto, E., Li, J., Zhou, B., Paul, A., Schwartz, R. J., Firulli, A. B., Singer, H. A., Huang, G., & Wu, M. (2016). Single-Cell Lineage Tracing Reveals that Oriented Cell Division Contributes to Trabecular Morphogenesis and Regional Specification. Cell Reports, 15(1), 158-170. https://doi.org/10.1016/j.celrep.2016.03.012