Single-particle tracking demonstrates that actin coordinates the movement of the Ebola virus matrix protein

Emmanuel Adu-Gyamfi, Michelle A. Digman, Enrico Gratton, Robert V. Stahelin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The Ebola virus causes severe hemorrhagic fever and has a mortality rate that can be as high as 90%, yet no vaccines or approved therapeutics, to our knowledge, are available. To replicate and egress the infected host cell the Ebola virus uses VP40, its major matrix protein to assemble at the inner leaflet of the plasma membrane. The assembly and budding of VP40 from the plasma membrane of host cells seem still poorly understood. We investigated the assembly and egress of VP40 at the plasma membrane of human cells using single-particle tracking. Our results demonstrate that actin coordinates the movement and assembly of VP40, a critical step in viral egress. These findings underscore the ability of single-molecule techniques to investigate the interplay of VP40 and host proteins in viral replication.

Original languageEnglish (US)
Pages (from-to)L41-L43
JournalBiophysical Journal
Volume103
Issue number9
DOIs
StatePublished - Nov 7 2012

Fingerprint

Ebolavirus
Actins
Cell Membrane
Proteins
Viral Proteins
Fever
Vaccines
Mortality
Therapeutics

ASJC Scopus subject areas

  • Biophysics

Cite this

Single-particle tracking demonstrates that actin coordinates the movement of the Ebola virus matrix protein. / Adu-Gyamfi, Emmanuel; Digman, Michelle A.; Gratton, Enrico; Stahelin, Robert V.

In: Biophysical Journal, Vol. 103, No. 9, 07.11.2012, p. L41-L43.

Research output: Contribution to journalArticle

Adu-Gyamfi, Emmanuel ; Digman, Michelle A. ; Gratton, Enrico ; Stahelin, Robert V. / Single-particle tracking demonstrates that actin coordinates the movement of the Ebola virus matrix protein. In: Biophysical Journal. 2012 ; Vol. 103, No. 9. pp. L41-L43.
@article{3d71af3a3e194b02a34135082412a761,
title = "Single-particle tracking demonstrates that actin coordinates the movement of the Ebola virus matrix protein",
abstract = "The Ebola virus causes severe hemorrhagic fever and has a mortality rate that can be as high as 90{\%}, yet no vaccines or approved therapeutics, to our knowledge, are available. To replicate and egress the infected host cell the Ebola virus uses VP40, its major matrix protein to assemble at the inner leaflet of the plasma membrane. The assembly and budding of VP40 from the plasma membrane of host cells seem still poorly understood. We investigated the assembly and egress of VP40 at the plasma membrane of human cells using single-particle tracking. Our results demonstrate that actin coordinates the movement and assembly of VP40, a critical step in viral egress. These findings underscore the ability of single-molecule techniques to investigate the interplay of VP40 and host proteins in viral replication.",
author = "Emmanuel Adu-Gyamfi and Digman, {Michelle A.} and Enrico Gratton and Stahelin, {Robert V.}",
year = "2012",
month = "11",
day = "7",
doi = "10.1016/j.bpj.2012.09.026",
language = "English (US)",
volume = "103",
pages = "L41--L43",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "9",

}

TY - JOUR

T1 - Single-particle tracking demonstrates that actin coordinates the movement of the Ebola virus matrix protein

AU - Adu-Gyamfi, Emmanuel

AU - Digman, Michelle A.

AU - Gratton, Enrico

AU - Stahelin, Robert V.

PY - 2012/11/7

Y1 - 2012/11/7

N2 - The Ebola virus causes severe hemorrhagic fever and has a mortality rate that can be as high as 90%, yet no vaccines or approved therapeutics, to our knowledge, are available. To replicate and egress the infected host cell the Ebola virus uses VP40, its major matrix protein to assemble at the inner leaflet of the plasma membrane. The assembly and budding of VP40 from the plasma membrane of host cells seem still poorly understood. We investigated the assembly and egress of VP40 at the plasma membrane of human cells using single-particle tracking. Our results demonstrate that actin coordinates the movement and assembly of VP40, a critical step in viral egress. These findings underscore the ability of single-molecule techniques to investigate the interplay of VP40 and host proteins in viral replication.

AB - The Ebola virus causes severe hemorrhagic fever and has a mortality rate that can be as high as 90%, yet no vaccines or approved therapeutics, to our knowledge, are available. To replicate and egress the infected host cell the Ebola virus uses VP40, its major matrix protein to assemble at the inner leaflet of the plasma membrane. The assembly and budding of VP40 from the plasma membrane of host cells seem still poorly understood. We investigated the assembly and egress of VP40 at the plasma membrane of human cells using single-particle tracking. Our results demonstrate that actin coordinates the movement and assembly of VP40, a critical step in viral egress. These findings underscore the ability of single-molecule techniques to investigate the interplay of VP40 and host proteins in viral replication.

UR - http://www.scopus.com/inward/record.url?scp=84868557277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868557277&partnerID=8YFLogxK

U2 - 10.1016/j.bpj.2012.09.026

DO - 10.1016/j.bpj.2012.09.026

M3 - Article

C2 - 23199932

AN - SCOPUS:84868557277

VL - 103

SP - L41-L43

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 9

ER -