SiRNA Delivery Using Dithiocarbamate-Anchored Oligonucleotides on Gold Nanorods

Jianxin Wang, Mini Thomas, Peng Lin, Ji Xin Cheng, Daniela Matei, Alexander Wei

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We present a robust method for loading small interfering RNA (siRNA) duplexes onto the surfaces of gold nanorods (GNRs) at high density, using near-infrared laser irradiation to trigger their intracellular release with subsequent knockdown activity. Citrate-stabilized GNRs were first coated with oleylsulfobetaine, a zwitterionic amphiphile with low cytotoxicity, which produced stable dispersions at high ionic strength. Amine-modified siRNA duplexes were converted into dithiocarbamate (DTC) ligands and adsorbed onto GNR surfaces in a single incubation step at 0.5 M NaCl, simplifying the charge screening process. The DTC anchors were effective at minimizing premature siRNA desorption and release, a common but often overlooked problem in the use of gold nanoparticles as oligonucleotide carriers. The activity of GNR-siRNA complexes was evaluated systematically against an eGFP-producing ovarian cancer cell line (SKOV-3) using folate receptor-mediated uptake. Efficient knockdown was achieved by using a femtosecond-pulsed laser source to release DTC-anchored siRNA, with essentially no contributions from spontaneous (dark) RNA desorption. GNRs coated with thiol-anchored siRNA duplexes were less effective and also permitted low levels of knockdown activity without photothermal activation. Optimized siRNA delivery conditions were applied toward the targeted knockdown of transglutaminase 2, whose expression is associated with the progression of recurrent ovarian cancer, with a reduction in activity of >80% achieved after a single pulsed laser treatment.

Original languageEnglish (US)
Pages (from-to)443-453
Number of pages11
JournalBioconjugate Chemistry
Volume30
Issue number2
DOIs
StatePublished - Feb 20 2019
Externally publishedYes

Fingerprint

Nanotubes
Oligonucleotides
RNA
Nanorods
Gold
Small Interfering RNA
Lasers
Pulsed lasers
Ovarian Neoplasms
Desorption
Amphiphiles
Infrared lasers
Laser beam effects
Cytotoxicity
Ionic strength
Anchors
Dispersions
Folic Acid
Sulfhydryl Compounds
Citric Acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

SiRNA Delivery Using Dithiocarbamate-Anchored Oligonucleotides on Gold Nanorods. / Wang, Jianxin; Thomas, Mini; Lin, Peng; Cheng, Ji Xin; Matei, Daniela; Wei, Alexander.

In: Bioconjugate Chemistry, Vol. 30, No. 2, 20.02.2019, p. 443-453.

Research output: Contribution to journalArticle

Wang, Jianxin ; Thomas, Mini ; Lin, Peng ; Cheng, Ji Xin ; Matei, Daniela ; Wei, Alexander. / SiRNA Delivery Using Dithiocarbamate-Anchored Oligonucleotides on Gold Nanorods. In: Bioconjugate Chemistry. 2019 ; Vol. 30, No. 2. pp. 443-453.
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