Site of action of atractyloside in mitochondria. II. Inhibition of oxidative phosphorylation

D. W. Allmann, R. A. Harris, D. E. Green

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The site of inhibition by atractyloside of oxidative phosphorylation and related processes in mitochondria prepared from beef heart was localized in the outer membrane. Support for this conclusion came from several lines of evidence: (a) the outer membrane is impenetrable to charged molecules of the size of acyl-CoA esters, adenine nucleotides, and pyridine nucleotides; (b) up to 60% of the total adenine nucleotides is localized in the outer membrane; (c) the release of AMP bound to the outer membrane is mediated by atractyloside; (d) exogenous adenine nucleotides can be bound by the outer membrane; (e) the loss of atractyloside inhibition parallels the loss (or alteration) of the outer membrane; (f) oxidative phosphorylation in submitochondrial particles such as ETPH is insensitive to atractyloside; and (g) the outer membrane contains a phosphoryl transferase (nucleoside monophosphokinase) which is atractyloside-sensitive.

Original languageEnglish (US)
Pages (from-to)766-782
Number of pages17
JournalArchives of Biochemistry and Biophysics
Volume122
Issue number3
DOIs
StatePublished - Dec 1967
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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