SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box

Bai Chang, Sen Partha, Kay Hofmann, Ma Lei, Mark Goebl, J. Wade Harper, Stephen J. Elledge

Research output: Contribution to journalArticle

849 Citations (Scopus)

Abstract

We have identified the yeast and human homologs of the SKP1 gene as a suppressor of cdc4 mutants and as a cyclin F-binding protein. Skp1p indirectly binds cyclin A/Cdk2 through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural motif called the F-box. SKP1 is required for ubiquitin-mediated proteolysis of Cln2p, Clb5p, and the Cdk inhibitor Sic1p, and provides a link between these molecules and the proteolysis machinery. A large number of proteins contain the F-box motif and are thereby implicated in the ubiquitin pathway. Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle.

Original languageEnglish
Pages (from-to)263-274
Number of pages12
JournalCell
Volume86
Issue number2
DOIs
StatePublished - Jul 26 1996

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F-Box Motifs
Proteolysis
Ubiquitin
Machinery
Cell Cycle
Cyclins
Cells
Cyclin A
Yeast
Carrier Proteins
Genes
Yeasts
Molecules
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. / Chang, Bai; Partha, Sen; Hofmann, Kay; Lei, Ma; Goebl, Mark; Harper, J. Wade; Elledge, Stephen J.

In: Cell, Vol. 86, No. 2, 26.07.1996, p. 263-274.

Research output: Contribution to journalArticle

Chang, Bai ; Partha, Sen ; Hofmann, Kay ; Lei, Ma ; Goebl, Mark ; Harper, J. Wade ; Elledge, Stephen J. / SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. In: Cell. 1996 ; Vol. 86, No. 2. pp. 263-274.
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AU - Harper, J. Wade

AU - Elledge, Stephen J.

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