Smad6s regulates plasminogen activator inhibitor-1 through a protein kinase C-β-dependent up-regulation of transforming growth factor-β

David T. Berg, Laura J. Myers, Mark A. Richardson, George Sandusky, Brian W. Grinnell

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Plasminogen activator inhibitor-1 (PAI-1) is a serpin class protease inhibitor that plays a central role in the regulation of vascular function and tissue remodeling by modulating thrombosis, inflammation, and the extracellular matrix. A central mediator controlling PAI-1 is transforming growth factor-β (TGF-β), which induces its expression and promotes fibrosis. We have found that a unique member of the Smad family of signal transduction molecules, Smad6s, modulates the expression of PAI-1. Overexpression of Smad6s in endothelial cells increases promoter activity and PAI-1 secretion, and an antisense to Smad6s suppresses the induction of PAI-1 by TGF-β. The effect of Smad6s on the PAI-1 promoter appeared to be the result of increase binding of the forkhead winged helix factor FoxD1 to a TGF-β-responsive element. Furthermore, the effect of Smad6s on PAI-1 up-regulation and on FoxD1 binding was found to result from up-regulation of TGF-β and could be inhibited by the blocking TGF-β signaling with Smad7. The ability of Smad6s to regulate the TGF-β promoter and subsequent PAI-1 induction was suppressed by a selective protein kinase C-β (PKC-β) inhibitor. Consistent with the in vitro data, we found that increased Smad6s in diseased vessels correlated with increased TGF-β and PAI-1 levels. Overall, our results demonstrate that the level of Smad6s can alter the level of TGF-β and the subsequent induction of PAI-1 via a FoxD1 transcription site. Furthermore, our data suggest that this process, which is up-regulated in diseased vessels, can be modulated by the inhibition of PKC-β.

Original languageEnglish (US)
Pages (from-to)14943-14947
Number of pages5
JournalJournal of Biological Chemistry
Volume280
Issue number15
DOIs
StatePublished - Apr 15 2005
Externally publishedYes

Fingerprint

Plasminogen Activator Inhibitor 1
Transforming Growth Factors
Protein Kinase C
Up-Regulation
Serpins
Signal transduction
Aptitude
Protein C Inhibitor
Endothelial cells
Transcription
Protein Kinase Inhibitors
Protease Inhibitors
Extracellular Matrix
Blood Vessels
Signal Transduction
Thrombosis
Fibrosis
Endothelial Cells
Tissue
Inflammation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Smad6s regulates plasminogen activator inhibitor-1 through a protein kinase C-β-dependent up-regulation of transforming growth factor-β. / Berg, David T.; Myers, Laura J.; Richardson, Mark A.; Sandusky, George; Grinnell, Brian W.

In: Journal of Biological Chemistry, Vol. 280, No. 15, 15.04.2005, p. 14943-14947.

Research output: Contribution to journalArticle

Berg, David T. ; Myers, Laura J. ; Richardson, Mark A. ; Sandusky, George ; Grinnell, Brian W. / Smad6s regulates plasminogen activator inhibitor-1 through a protein kinase C-β-dependent up-regulation of transforming growth factor-β. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 15. pp. 14943-14947.
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abstract = "Plasminogen activator inhibitor-1 (PAI-1) is a serpin class protease inhibitor that plays a central role in the regulation of vascular function and tissue remodeling by modulating thrombosis, inflammation, and the extracellular matrix. A central mediator controlling PAI-1 is transforming growth factor-β (TGF-β), which induces its expression and promotes fibrosis. We have found that a unique member of the Smad family of signal transduction molecules, Smad6s, modulates the expression of PAI-1. Overexpression of Smad6s in endothelial cells increases promoter activity and PAI-1 secretion, and an antisense to Smad6s suppresses the induction of PAI-1 by TGF-β. The effect of Smad6s on the PAI-1 promoter appeared to be the result of increase binding of the forkhead winged helix factor FoxD1 to a TGF-β-responsive element. Furthermore, the effect of Smad6s on PAI-1 up-regulation and on FoxD1 binding was found to result from up-regulation of TGF-β and could be inhibited by the blocking TGF-β signaling with Smad7. The ability of Smad6s to regulate the TGF-β promoter and subsequent PAI-1 induction was suppressed by a selective protein kinase C-β (PKC-β) inhibitor. Consistent with the in vitro data, we found that increased Smad6s in diseased vessels correlated with increased TGF-β and PAI-1 levels. Overall, our results demonstrate that the level of Smad6s can alter the level of TGF-β and the subsequent induction of PAI-1 via a FoxD1 transcription site. Furthermore, our data suggest that this process, which is up-regulated in diseased vessels, can be modulated by the inhibition of PKC-β.",
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