Small-molecule inhibitor screen for DNA repair proteins

John J. Turchi, Pamela S. VanderVere-Carozza

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

With the recent interest in targeting the DNA damage response (DDR) and DNA repair, new screening methodologies are needed to broaden the scope of targetable proteins beyond kinases and traditional enzymes. Many of the proteins involved in the DDR and repair impart their activity by making specific contacts with DNA. These protein–nucleic acid interactions represent a tractable target for perturbation with small molecules. We describe a high throughput, solution-based equilibrium binding fluorescence polarization assay that can be applied to a wide array of protein–nucleic acid interactions. The assay is sensitive, stable, and able to identify small molecules capable of blocking DNA–protein interactions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages217-221
Number of pages5
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1999
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • DNA damage response
  • DNA repair
  • Fluorescence polarization
  • High-throughput screening
  • Nucleic acid enzymology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Turchi, J. J., & VanderVere-Carozza, P. S. (2019). Small-molecule inhibitor screen for DNA repair proteins. In Methods in Molecular Biology (pp. 217-221). (Methods in Molecular Biology; Vol. 1999). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9500-4_13