Small-molecule inhibitor screen for DNA repair proteins

John Turchi, Pamela S. VanderVere-Carozza

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

With the recent interest in targeting the DNA damage response (DDR) and DNA repair, new screening methodologies are needed to broaden the scope of targetable proteins beyond kinases and traditional enzymes. Many of the proteins involved in the DDR and repair impart their activity by making specific contacts with DNA. These protein–nucleic acid interactions represent a tractable target for perturbation with small molecules. We describe a high throughput, solution-based equilibrium binding fluorescence polarization assay that can be applied to a wide array of protein–nucleic acid interactions. The assay is sensitive, stable, and able to identify small molecules capable of blocking DNA–protein interactions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages217-221
Number of pages5
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1999
ISSN (Print)1064-3745

Fingerprint

DNA Repair
DNA Damage
Fluorescence Polarization
Acids
Protein Kinases
Proteins
DNA
Enzymes

Keywords

  • DNA damage response
  • DNA repair
  • Fluorescence polarization
  • High-throughput screening
  • Nucleic acid enzymology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Turchi, J., & VanderVere-Carozza, P. S. (2019). Small-molecule inhibitor screen for DNA repair proteins. In Methods in Molecular Biology (pp. 217-221). (Methods in Molecular Biology; Vol. 1999). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9500-4_13

Small-molecule inhibitor screen for DNA repair proteins. / Turchi, John; VanderVere-Carozza, Pamela S.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 217-221 (Methods in Molecular Biology; Vol. 1999).

Research output: Chapter in Book/Report/Conference proceedingChapter

Turchi, J & VanderVere-Carozza, PS 2019, Small-molecule inhibitor screen for DNA repair proteins. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1999, Humana Press Inc., pp. 217-221. https://doi.org/10.1007/978-1-4939-9500-4_13
Turchi J, VanderVere-Carozza PS. Small-molecule inhibitor screen for DNA repair proteins. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 217-221. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9500-4_13
Turchi, John ; VanderVere-Carozza, Pamela S. / Small-molecule inhibitor screen for DNA repair proteins. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 217-221 (Methods in Molecular Biology).
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