Small molecule target identification using photo-affinity chromatography

Seung Yong Seo, Timothy W. Corson

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Identification of the protein targets of bioactive small molecules is a routine challenge in chemical biology and phenotype-based drug discovery. Recent years have seen an explosion of approaches to meeting this challenge, but the traditional method of affinity pulldowns remains a practical choice in many contexts. This technique can be used as long as an affinity probe can be synthesized, usually with a crosslinking moiety to enable photo-affinity pulldowns. It can be applied to varied tissue types and can be performed with minimal specialized equipment. Here, we provide our protocol for photo-affinity pulldown experiments, with notes on making this method generally applicable to varied target identification challenges.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsArun K. Shukla
PublisherAcademic Press Inc.
Number of pages28
ISBN (Print)9780128181195
StatePublished - 2019

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Biotin
  • Click chemistry
  • Inhibitor
  • Mass spectrometry
  • Natural product
  • Off-target
  • Photoreactive group
  • Small molecule
  • Synthetic chemistry
  • Target identification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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