Small molecule target identification using photo-affinity chromatography

Seung Yong Seo, Timothy Corson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Identification of the protein targets of bioactive small molecules is a routine challenge in chemical biology and phenotype-based drug discovery. Recent years have seen an explosion of approaches to meeting this challenge, but the traditional method of affinity pulldowns remains a practical choice in many contexts. This technique can be used as long as an affinity probe can be synthesized, usually with a crosslinking moiety to enable photo-affinity pulldowns. It can be applied to varied tissue types and can be performed with minimal specialized equipment. Here, we provide our protocol for photo-affinity pulldown experiments, with notes on making this method generally applicable to varied target identification challenges.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Affinity chromatography
Affinity Chromatography
Crosslinking
Explosions
Tissue
Molecules
Drug Discovery
Proteins
Experiments
Phenotype
Equipment and Supplies

Keywords

  • Biotin
  • Click chemistry
  • Inhibitor
  • Mass spectrometry
  • Natural product
  • Off-target
  • Photoreactive group
  • Small molecule
  • Synthetic chemistry
  • Target identification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Seo, S. Y., & Corson, T. (2019). Small molecule target identification using photo-affinity chromatography. In Methods in Enzymology (Methods in Enzymology). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2019.02.028

Small molecule target identification using photo-affinity chromatography. / Seo, Seung Yong; Corson, Timothy.

Methods in Enzymology. Academic Press Inc., 2019. (Methods in Enzymology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Seo, SY & Corson, T 2019, Small molecule target identification using photo-affinity chromatography. in Methods in Enzymology. Methods in Enzymology, Academic Press Inc. https://doi.org/10.1016/bs.mie.2019.02.028
Seo SY, Corson T. Small molecule target identification using photo-affinity chromatography. In Methods in Enzymology. Academic Press Inc. 2019. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2019.02.028
Seo, Seung Yong ; Corson, Timothy. / Small molecule target identification using photo-affinity chromatography. Methods in Enzymology. Academic Press Inc., 2019. (Methods in Enzymology).
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