SNS Na+ channel expression increases in dorsal root ganglion neurons in the carrageenan inflammatory pain model

Masaki Tanaka, Theodore R. Cummins, Kuniko Ishikawa, Sulayman D. Dib-Hajj, Joel A. Black, Stephen G. Waxman

Research output: Contribution to journalArticlepeer-review

203 Scopus citations

Abstract

It has been suggested that hyperexcitability in dorsal root ganglion (DRG) neurons due to altered sodium channel expression contributes to some chronic pain syndromes. To understand the role of the voltage-gated sodium channel α-SNS in inflammatory pain, we investigated the expression of α- SNS mRNA and tetrodotoxin-resistant (TTX-R) sodium current in small DRG neurons, which include nociceptive cells, following injection of carrageenan into the hind paw of the rat using in situ hybridization and patch-clamp recording. α-SNS mRNA expression in DRG neurons projecting to the inflamed limb was significantly increased 4 days following carrageenan injection, compared with DRG neurons from the contralateral side or naive (uninjected) rats (mean±s.d. optical density ratio: ipsilateral/contralateral, 1.77 ± 0.17; ipsilateral/naive, 1.88 ± 0.36). The amplitude of the TTX-R sodium current in small DRG neurons projecting to the inflamed limb was significantly larger than on the contralateral side 4 days post-injection (31.7 ± 3.3 vs 20.0 ± 2.1 nA). The TTX-R current density was also significantly increased. These results demonstrate the increased expression of α-SNS sodium channels in small DRG neurons following injection of carrageenan into their projection field, and suggest that α-SNS is involved in the development of hyperexcitability associated with inflammation.

Original languageEnglish (US)
Pages (from-to)967-972
Number of pages6
JournalNeuroReport
Volume9
Issue number6
DOIs
StatePublished - Apr 20 1998
Externally publishedYes

Keywords

  • Carrageenan
  • Dorsal root ganglion
  • In situ hybridization
  • Inflammatory pain
  • Rat
  • Whole-cell patch-clamp
  • α-SNS sodium channel

ASJC Scopus subject areas

  • Neuroscience(all)

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