Soft tissue calcification in the Ossabaw miniature pig: Experimental and kinetic modeling studies

M. Wastney, W. Lee, G. S. Jackson, Mouhamad Alloosh, Michael Sturek, P. Lachcik, Munro Peacock, B. Martin, C. M. Weaver

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Calcium (Ca) deposition into vascular tissue was measured in Ossabaw miniature pigs with and without metabolic syndrome (MetS) using Ca tracer kinetics and coronary atherosclerosis measured with intravascular ultrasound. Pigs with MetS had higher Ca uptake into coronary arteries than lean pigs. Introduction: Ca deposition into arteries is a common disease in humans. The Ossabaw pig develops MetS when fed an atherogenic diet. The aim of this study was to measure Ca deposition into arteries of lean vs. MetS pigs. Methods: Male pigs were fed for 5 months with chow diet (healthy, lean; n = 7) or atherogenic diet (n = 8) consisting of chow supplemented with 2 % cholesterol, 43 % kcal from fat, and 20 % kcal from fructose. Pigs were verified to have MetS by obesity, insulin resistance, impaired glucose tolerance, dyslipidemia, and hypertension. Two pigs received 50 nCi of 41Ca i.v. and blood was drawn frequently for 24 h, and 2, 3, 6, 8, 10, 15, 20, and at sacrifice at 28 days after injection. Peripheral arteries were biopsied four times per pig over the 28th day and coronary artery sampled at sacrifice. Tissues were analyzed for 41Ca:Ca. A compartmental model was used to estimate rates of Ca deposition into the arteries. Results: The MetS swine had higher 41Ca and atherosclerosis in coronary arteries than lean pigs. Conclusions: This pig model is a suitable model for studying vascular calcification in humans.

Original languageEnglish
Pages (from-to)2123-2126
Number of pages4
JournalOsteoporosis International
Volume24
Issue number7
DOIs
StatePublished - Jul 2013

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Swine
Calcium
Arteries
Atherogenic Diet
Coronary Vessels
Vascular Calcification
Glucose Intolerance
Dyslipidemias
Fructose
Blood Vessels
Insulin Resistance
Coronary Artery Disease
Atherosclerosis
Obesity
Fats
Cholesterol
Hypertension
Injections

Keywords

  • Ca
  • Metabolic syndrome
  • Pig
  • Vascular calcification

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Cite this

Soft tissue calcification in the Ossabaw miniature pig : Experimental and kinetic modeling studies. / Wastney, M.; Lee, W.; Jackson, G. S.; Alloosh, Mouhamad; Sturek, Michael; Lachcik, P.; Peacock, Munro; Martin, B.; Weaver, C. M.

In: Osteoporosis International, Vol. 24, No. 7, 07.2013, p. 2123-2126.

Research output: Contribution to journalArticle

Wastney, M. ; Lee, W. ; Jackson, G. S. ; Alloosh, Mouhamad ; Sturek, Michael ; Lachcik, P. ; Peacock, Munro ; Martin, B. ; Weaver, C. M. / Soft tissue calcification in the Ossabaw miniature pig : Experimental and kinetic modeling studies. In: Osteoporosis International. 2013 ; Vol. 24, No. 7. pp. 2123-2126.
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AB - Calcium (Ca) deposition into vascular tissue was measured in Ossabaw miniature pigs with and without metabolic syndrome (MetS) using Ca tracer kinetics and coronary atherosclerosis measured with intravascular ultrasound. Pigs with MetS had higher Ca uptake into coronary arteries than lean pigs. Introduction: Ca deposition into arteries is a common disease in humans. The Ossabaw pig develops MetS when fed an atherogenic diet. The aim of this study was to measure Ca deposition into arteries of lean vs. MetS pigs. Methods: Male pigs were fed for 5 months with chow diet (healthy, lean; n = 7) or atherogenic diet (n = 8) consisting of chow supplemented with 2 % cholesterol, 43 % kcal from fat, and 20 % kcal from fructose. Pigs were verified to have MetS by obesity, insulin resistance, impaired glucose tolerance, dyslipidemia, and hypertension. Two pigs received 50 nCi of 41Ca i.v. and blood was drawn frequently for 24 h, and 2, 3, 6, 8, 10, 15, 20, and at sacrifice at 28 days after injection. Peripheral arteries were biopsied four times per pig over the 28th day and coronary artery sampled at sacrifice. Tissues were analyzed for 41Ca:Ca. A compartmental model was used to estimate rates of Ca deposition into the arteries. Results: The MetS swine had higher 41Ca and atherosclerosis in coronary arteries than lean pigs. Conclusions: This pig model is a suitable model for studying vascular calcification in humans.

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