Soleus muscle atrophy in rats induced by cast immobilization: Lack of effect by anabolic steroids

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Abstract

To test the hypothesis that an anabolic steroid such as nandrolone decanoate (ND) will ameliorate or abolish disuse-mediated alterations resulting from five weeks of hindlimb immobilization, female Sprague-Dawley rats were divided into six groups: control, control + ND, shortened-immobilized, shortened-immobilized + ND, stretched-immobilized, and stretched-immobilized + ND. Immobilization was accomplished by wrapping hindlimbs with plaster of Paris. Nandrolone decanoate in sesame oil was administered via weekly intraperitoneal injection (7 mg/kg) while nontreated rats received equivalent volumes of the vehicle. After five weeks selected morphometric, biochemical, and mechanical parameters were examined in the slow-twitch soleus muscle (SOL). Muscle wet weight fell from a control value of 145 ± 19 mg to 70 ± 8 mg (p <.05) in the immobilized-shortened group where ND had no effect (81 ± 11 mg). Muscle stretch alone prevented weight losS (159 ± 31 mg). The addition of ND resulted in significant SOL hypertrophy (200 ± 42 mg), p <.05. Immobilization resulted in a significant shift in muscle protein distribution toward sarcoplasmic protein, a change unaltered by ND but abolished by stretch. Muscle strength, as indicated by peak tetanic tension, fell 45% (p <.05) as a result of shortened immobilization. Nandrolone decanoate had no effect on this condition, although the ameliorating effect of stretch was abolished when in combinaton with ND treatment. The elevation observed in maximal shortening velocity (shortened-immobilized) was unaffected by ND alone, but abolished by stretch with or without ND treatment. These results demonstrate the absence of therapeutic effect when ND is administered during muscle atrophy. Furthermore, under the experimental conditions described, ND in combination with chronic immobilized stretch significantly impairs tetanic tension generation in the predominantly slow-twitch SOL.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalArchives of Physical Medicine and Rehabilitation
Volume69
Issue number2
StatePublished - 1988
Externally publishedYes

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Testosterone Congeners
Muscular Atrophy
Immobilization
Skeletal Muscle
nandrolone decanoate
Sesame Oil
Hindlimb Suspension
Calcium Sulfate
Muscles
Muscle Proteins
Muscle Strength

ASJC Scopus subject areas

  • Rehabilitation

Cite this

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title = "Soleus muscle atrophy in rats induced by cast immobilization: Lack of effect by anabolic steroids",
abstract = "To test the hypothesis that an anabolic steroid such as nandrolone decanoate (ND) will ameliorate or abolish disuse-mediated alterations resulting from five weeks of hindlimb immobilization, female Sprague-Dawley rats were divided into six groups: control, control + ND, shortened-immobilized, shortened-immobilized + ND, stretched-immobilized, and stretched-immobilized + ND. Immobilization was accomplished by wrapping hindlimbs with plaster of Paris. Nandrolone decanoate in sesame oil was administered via weekly intraperitoneal injection (7 mg/kg) while nontreated rats received equivalent volumes of the vehicle. After five weeks selected morphometric, biochemical, and mechanical parameters were examined in the slow-twitch soleus muscle (SOL). Muscle wet weight fell from a control value of 145 ± 19 mg to 70 ± 8 mg (p <.05) in the immobilized-shortened group where ND had no effect (81 ± 11 mg). Muscle stretch alone prevented weight losS (159 ± 31 mg). The addition of ND resulted in significant SOL hypertrophy (200 ± 42 mg), p <.05. Immobilization resulted in a significant shift in muscle protein distribution toward sarcoplasmic protein, a change unaltered by ND but abolished by stretch. Muscle strength, as indicated by peak tetanic tension, fell 45{\%} (p <.05) as a result of shortened immobilization. Nandrolone decanoate had no effect on this condition, although the ameliorating effect of stretch was abolished when in combinaton with ND treatment. The elevation observed in maximal shortening velocity (shortened-immobilized) was unaffected by ND alone, but abolished by stretch with or without ND treatment. These results demonstrate the absence of therapeutic effect when ND is administered during muscle atrophy. Furthermore, under the experimental conditions described, ND in combination with chronic immobilized stretch significantly impairs tetanic tension generation in the predominantly slow-twitch SOL.",
author = "Frank Witzmann",
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T1 - Soleus muscle atrophy in rats induced by cast immobilization

T2 - Lack of effect by anabolic steroids

AU - Witzmann, Frank

PY - 1988

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