Solubilization of hormone-responsive adenylate cyclase from human renal cortex

Frederick G. Hamel, Sherry F. Queener

Research output: Contribution to journalArticle

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Abstract

Adenylate cyclase activity from human renal cortical plasma membranes remained in the 100,000 xg supernatant (2 hrs) following treatment with 0.25% Lubrol PX in 10mM Tris buffer (pH 7.45), 1 mM EDTA, 0.25 M sucrose, and 5 mM NaF. Solubilization decreased total adenylate cyclase activity by at least one-half; responsiveness to calcitonin, glucagon and guanyl nucleotides, but not to parathyroid hormone, was preserved. Glucagon and calcitonin-stimulated adenylate cyclase eluted near the void volume on Sephadex G200 columns; two other peaks of non-hormone stimulated activity eluted later.

Original languageEnglish (US)
Pages (from-to)567-575
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume108
Issue number2
DOIs
StatePublished - Sep 30 1982

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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