Soluble and bound acid protease activity of myelin from bovine cerebral white matter and spinal cord

Hans H. Berlet, Heike Ilzenhöfer, Martin Kaefer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Isolated myelin of bovine spinal cord was found to degrade exogenous myelin basic protein (MBP) at pH 4.4. Electrophoretic peptide patterns were consistent with limited proteolysis of MBP. Some of the proteolytic activity was soluble at increased ionic strength, some remained bound, withstanding extraction at 37°C for up to 12 hr. While being measurable with exogenous MBP, bound protease degraded neither bound MBP nor any other major intrinsic myelin protein. Both soluble and bound protease activity was completely inhibited by pepstatin A. The patterns of limited proteolysis of MBP they produced were identical. Myelin of cerebral white matter also exhibited soluble and bound acid protease activity which was likewise inhibited by pepstatin A. Protease activity of spinal cord and cerebral myelin is therefore suggested to be due to a cathepsin D-like endopeptidase, present in a loosely and tightly bound form. Both forms increased by 50 to 80% in activity when myelin was isolated from mixtures of white and cortical gray matter. While increased soluble activity of myelin is consistent with binding of cathepsin D of lysosomal origin during the isolation of myelin the tightly bound form might point to a principal mechanism through which exogenous proteins may become attached to the myelin sheath in vivo.

Original languageEnglish (US)
Pages (from-to)409-416
Number of pages8
JournalNeurochemical Research
Volume13
Issue number5
DOIs
StatePublished - May 1988
Externally publishedYes

Fingerprint

Myelin Basic Protein
Myelin Sheath
Spinal Cord
Peptide Hydrolases
Acids
Proteolysis
Cathepsin D
Myelin Proteins
Endopeptidases
Ionic strength
White Matter
Osmolar Concentration
Peptides
Proteins

Keywords

  • Bovine central myelin
  • cathepsin D
  • limited proteolysis
  • myelin basic protein

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Soluble and bound acid protease activity of myelin from bovine cerebral white matter and spinal cord. / Berlet, Hans H.; Ilzenhöfer, Heike; Kaefer, Martin.

In: Neurochemical Research, Vol. 13, No. 5, 05.1988, p. 409-416.

Research output: Contribution to journalArticle

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