Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer

X. Gao, Y. Q. Chen, N. Wu, D. J. Grignon, W. Sakr, A. T. Porter, K. V. Honn

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

The WAF1/CIP1 gene, a potential tumor suppressor gene, has recently been cloned and identified as a p53 mediator and an inhibitor for G1 cyclin-dependent kinases (CDKs). We undertook this study to investigate the possible role of the WAF1/CIP1 gene in human prostatic carcinoma. Matched normal and cancer tissues from 18 patients with prostate cancer were screened for WAF1/CIP1 mutation by nested reverse transcription-polymerase chain reaction/single strand conformational polymorphism (RT-PCR/SSCP) and DNA sequencing. Shifted bands from three tumor, but not the matched normal specimens, were observed. Subsequent direct DNA sequencing of the PCR fragments identified four sequence alterations including a cytosine (C) to adenine (A) transversion and a guanine (G) to A transition and two A insertions. Our results demonstrated that mutations of the WAF1/CIP1 gene occur and may be important during the pathogenesis of human prostate cancer. This is the first report of WAF1/CIP1 mutation in a primary human cancer.

Original languageEnglish (US)
Pages (from-to)1395-1398
Number of pages4
JournalOncogene
Volume11
Issue number7
StatePublished - Jan 1 1995

Keywords

  • Mutation
  • Polymerase chain reaction
  • Prostatic adenocarcinoma
  • Tumor suppressor gene
  • WAF1/CIP1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Gao, X., Chen, Y. Q., Wu, N., Grignon, D. J., Sakr, W., Porter, A. T., & Honn, K. V. (1995). Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer. Oncogene, 11(7), 1395-1398.