Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer

X. Gao, Y. Q. Chen, N. Wu, David Grignon, W. Sakr, A. T. Porter, K. V. Honn

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The WAF1/CIP1 gene, a potential tumor suppressor gene, has recently been cloned and identified as a p53 mediator and an inhibitor for G1 cyclin-dependent kinases (CDKs). We undertook this study to investigate the possible role of the WAF1/CIP1 gene in human prostatic carcinoma. Matched normal and cancer tissues from 18 patients with prostate cancer were screened for WAF1/CIP1 mutation by nested reverse transcription-polymerase chain reaction/single strand conformational polymorphism (RT-PCR/SSCP) and DNA sequencing. Shifted bands from three tumor, but not the matched normal specimens, were observed. Subsequent direct DNA sequencing of the PCR fragments identified four sequence alterations including a cytosine (C) to adenine (A) transversion and a guanine (G) to A transition and two A insertions. Our results demonstrated that mutations of the WAF1/CIP1 gene occur and may be important during the pathogenesis of human prostate cancer. This is the first report of WAF1/CIP1 mutation in a primary human cancer.

Original languageEnglish (US)
Pages (from-to)1395-1398
Number of pages4
JournalOncogene
Volume11
Issue number7
StatePublished - 1995
Externally publishedYes

Fingerprint

Prostatic Neoplasms
DNA Sequence Analysis
Mutation
Genes
Neoplasms
Polymerase Chain Reaction
Cyclin-Dependent Kinases
Cytosine
Guanine
Adenine
Tumor Suppressor Genes
Reverse Transcription
Carcinoma

Keywords

  • Mutation
  • Polymerase chain reaction
  • Prostatic adenocarcinoma
  • Tumor suppressor gene
  • WAF1/CIP1

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Gao, X., Chen, Y. Q., Wu, N., Grignon, D., Sakr, W., Porter, A. T., & Honn, K. V. (1995). Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer. Oncogene, 11(7), 1395-1398.

Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer. / Gao, X.; Chen, Y. Q.; Wu, N.; Grignon, David; Sakr, W.; Porter, A. T.; Honn, K. V.

In: Oncogene, Vol. 11, No. 7, 1995, p. 1395-1398.

Research output: Contribution to journalArticle

Gao, X, Chen, YQ, Wu, N, Grignon, D, Sakr, W, Porter, AT & Honn, KV 1995, 'Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer', Oncogene, vol. 11, no. 7, pp. 1395-1398.
Gao X, Chen YQ, Wu N, Grignon D, Sakr W, Porter AT et al. Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer. Oncogene. 1995;11(7):1395-1398.
Gao, X. ; Chen, Y. Q. ; Wu, N. ; Grignon, David ; Sakr, W. ; Porter, A. T. ; Honn, K. V. / Somatic mutations of the WAF1/CIP1 gene in primary prostate cancer. In: Oncogene. 1995 ; Vol. 11, No. 7. pp. 1395-1398.
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