Somatic point mutation of the wild-type allele detected in tumors of patients with VHL germline deletion

Alexander Vortmeyer, Steve C. Huang, Svetlana D. Pack, Christian A. Koch, Irina A. Lubensky, Edward H. Oldfield, Zhengping Zhuang

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The majority of patients with Von Hippel-Lindau (VHL) disease are affected by a VHL germline mutation involving one copy of the VHL gene. Loss of heterozygosity of the second VHL allele can be consistently demonstrated in tumor tissue from these patients, suggesting that allelic deletion is a very early or even initiating event for tumorigenesis. Approximately 20% of VHL disease patients, however, exhibit germline deletion of one entire copy or at least a substantial part of the VHL gene. To investigate the nature of the 'second genetic hit' in this patient population, we analysed two renal cell carcinomas and one CNS hemangioblastoma from three unrelated patients for genetic changes of the second copy of the VHL gene. All three tumors showed retention of one VHL allele by FISH. Single-strand conformation polymorphism and mutation analysis of microdissected tumor DNA revealed somatic point mutations of the wild-type VHL copies in each of the three tumors. The results indicate that the 'two hit model' is equally applicable to patients with VHL germline mutation and VHL germline deletion. In contrast to tumors from patients with VHL germline mutation, however, point mutations of the wild-type allele can be detected in tumors from patients with VHL germline deletion.

Original languageEnglish (US)
Pages (from-to)1167-1170
Number of pages4
JournalOncogene
Volume21
Issue number8
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

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Keywords

  • Deletion
  • Mutation
  • VHL disease
  • VHL gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Vortmeyer, A., Huang, S. C., Pack, S. D., Koch, C. A., Lubensky, I. A., Oldfield, E. H., & Zhuang, Z. (2002). Somatic point mutation of the wild-type allele detected in tumors of patients with VHL germline deletion. Oncogene, 21(8), 1167-1170. https://doi.org/10.1038/sj.onc.1205121