Spatial and temporal regulation of coronary vessel formation by calcineurin-NFAT signaling

Miriam Zeini, Calvin T. Hang, Joshua Lehrer-Graiwer, Tiffany Dao, Bin Zhou, Ching-Pin Chang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Formation of the coronary vasculature requires reciprocal signaling between endothelial, epicardially derived smooth muscle and underlying myocardial cells. Our studies show that calcineurin-NFAT signaling functions in endothelial cells within specific time windows to regulate coronary vessel development. Mouse embryos exposed to cyclosporin A (CsA), which inhibits calcineurin phosphatase activity, failed to develop normal coronary vasculature. To determine the cellular site at which calcineurin functions for coronary angiogenesis, we deleted calcineurin in endothelial, epicardial and myocardial cells. Disruption of calcineurin-NFAT signaling in endothelial cells resulted in the failure of coronary angiogenesis, recapitulating the coronary phenotype observed in CsA-treated embryos. By contrast, deletion of calcineurin in either epicardial or myocardial cells had no effect on coronary vasculature during early embryogenesis. To define the temporal requirement for NFAT signaling, we treated developing embryos with CsA at overlapping windows from E9.5 to E12.5 and examined coronary development at E12.5. These experiments demonstrated that calcineurin-NFAT signaling functions between E10.5 and E11.5 to regulate coronary angiogenesis. Consistent with these in vivo observations, endothelial cells exposed to CsA within specific time windows in tissue culture were unable to form tubular structures and their cellular responses to VEGF-A were blunted. Thus, our studies demonstrate specific temporal and spatial requirements of NFAT signaling for coronary vessel angiogenesis. These requirements are distinct from the roles of NFAT signaling in the angiogenesis of peripheral somatic vessels, providing an example of the environmental influence of different vascular beds on the in vivo endothelial responses to angiogenic stimuli.

Original languageEnglish (US)
Pages (from-to)3335-3345
Number of pages11
JournalDevelopment (Cambridge)
Volume136
Issue number19
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

Fingerprint

Calcineurin
Coronary Vessels
Cyclosporine
Embryonic Structures
Endothelial Cells
Cellular Structures
Vascular Endothelial Growth Factor A
Embryonic Development
Smooth Muscle
Blood Vessels
Phenotype

Keywords

  • Calcineurin (PPP3CA; PPP3R1)
  • Coronary vessel
  • Endothelial cell
  • Heart development
  • Hedgehog
  • Mouse
  • NFAT
  • VEGF

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Zeini, M., Hang, C. T., Lehrer-Graiwer, J., Dao, T., Zhou, B., & Chang, C-P. (2009). Spatial and temporal regulation of coronary vessel formation by calcineurin-NFAT signaling. Development (Cambridge), 136(19), 3335-3345. https://doi.org/10.1242/dev.037903

Spatial and temporal regulation of coronary vessel formation by calcineurin-NFAT signaling. / Zeini, Miriam; Hang, Calvin T.; Lehrer-Graiwer, Joshua; Dao, Tiffany; Zhou, Bin; Chang, Ching-Pin.

In: Development (Cambridge), Vol. 136, No. 19, 01.10.2009, p. 3335-3345.

Research output: Contribution to journalArticle

Zeini, M, Hang, CT, Lehrer-Graiwer, J, Dao, T, Zhou, B & Chang, C-P 2009, 'Spatial and temporal regulation of coronary vessel formation by calcineurin-NFAT signaling', Development (Cambridge), vol. 136, no. 19, pp. 3335-3345. https://doi.org/10.1242/dev.037903
Zeini, Miriam ; Hang, Calvin T. ; Lehrer-Graiwer, Joshua ; Dao, Tiffany ; Zhou, Bin ; Chang, Ching-Pin. / Spatial and temporal regulation of coronary vessel formation by calcineurin-NFAT signaling. In: Development (Cambridge). 2009 ; Vol. 136, No. 19. pp. 3335-3345.
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