Spatial distribution of nerve sprouting after myocardial infarction in mice

Yong Seog Oh, Ambrose Y. Jong, Dave T. Kim, Hongmei Li, Charles Wang, Alice Zemljic-Harpf, Robert S. Ross, Michael C. Fishbein, Peng Sheng Chen, Lan S. Chen

Research output: Contribution to journalArticle

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Abstract

Background: Myocardial infarction (MI) elicits nerve sprouting. Objectives: The purpose of this study was to determine the spatial distribution of nerve sprouting and neurotrophic gene expression after MI. Methods: We created MI in mice by coronary artery ligation. The hearts were removed 3 hours to 2 months after MI and examined for nerve fiber density and neurotrophic factor gene expression using Affymetrix microarray and mRNA analyses. Results: The density of nerve fibers immunopositive for growth-associated protein (GAP)-43 was the highest 3 hours after MI both in the peri-infarct area and in the area remote to infarct, resulting in sympathetic (but not parasympathetic) hyperinnervation in the ventricles. The GAP-43-positive nerve fiber density of myocardium was greater in the outer transverse loop than in the inner vertical loop. The differences between these two myocardial loops peaked within 3 hours after MI and persisted for 2 months afterward. Gene expression of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α was increased up to 2 months after MI compared with normal control. Expression of these growth factors was more pronounced and persistent in the peri-infarct area than in the remote area. Conclusion: MI induces sympathetic nerve sprouting in both peri-infarct and remote areas, more in the outer transverse loop. Selective up-regulation of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α occurred in the peri-infarct area and, to a lesser extent, in the remote area.

Original languageEnglish (US)
Pages (from-to)728-736
Number of pages9
JournalHeart Rhythm
Volume3
Issue number6
DOIs
StatePublished - Jun 1 2006

Fingerprint

Myocardial Infarction
Nerve Fibers
GAP-43 Protein
Leukemia Inhibitory Factor
Transforming Growth Factors
Nerve Growth Factor
Somatomedins
Interleukin-1
Gene Expression
Nerve Growth Factors
Microarray Analysis
Ligation
Intercellular Signaling Peptides and Proteins
Coronary Vessels
Myocardium
Up-Regulation
Messenger RNA

Keywords

  • Cholinergic nerve
  • DNA microarray
  • Helical heart
  • Myocardial infarction
  • Nerve sprouting
  • Neural regeneration
  • Neural remodeling
  • Neurotrophic factors
  • Sympathetic nerve

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Oh, Y. S., Jong, A. Y., Kim, D. T., Li, H., Wang, C., Zemljic-Harpf, A., ... Chen, L. S. (2006). Spatial distribution of nerve sprouting after myocardial infarction in mice. Heart Rhythm, 3(6), 728-736. https://doi.org/10.1016/j.hrthm.2006.02.005

Spatial distribution of nerve sprouting after myocardial infarction in mice. / Oh, Yong Seog; Jong, Ambrose Y.; Kim, Dave T.; Li, Hongmei; Wang, Charles; Zemljic-Harpf, Alice; Ross, Robert S.; Fishbein, Michael C.; Chen, Peng Sheng; Chen, Lan S.

In: Heart Rhythm, Vol. 3, No. 6, 01.06.2006, p. 728-736.

Research output: Contribution to journalArticle

Oh, YS, Jong, AY, Kim, DT, Li, H, Wang, C, Zemljic-Harpf, A, Ross, RS, Fishbein, MC, Chen, PS & Chen, LS 2006, 'Spatial distribution of nerve sprouting after myocardial infarction in mice', Heart Rhythm, vol. 3, no. 6, pp. 728-736. https://doi.org/10.1016/j.hrthm.2006.02.005
Oh YS, Jong AY, Kim DT, Li H, Wang C, Zemljic-Harpf A et al. Spatial distribution of nerve sprouting after myocardial infarction in mice. Heart Rhythm. 2006 Jun 1;3(6):728-736. https://doi.org/10.1016/j.hrthm.2006.02.005
Oh, Yong Seog ; Jong, Ambrose Y. ; Kim, Dave T. ; Li, Hongmei ; Wang, Charles ; Zemljic-Harpf, Alice ; Ross, Robert S. ; Fishbein, Michael C. ; Chen, Peng Sheng ; Chen, Lan S. / Spatial distribution of nerve sprouting after myocardial infarction in mice. In: Heart Rhythm. 2006 ; Vol. 3, No. 6. pp. 728-736.
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abstract = "Background: Myocardial infarction (MI) elicits nerve sprouting. Objectives: The purpose of this study was to determine the spatial distribution of nerve sprouting and neurotrophic gene expression after MI. Methods: We created MI in mice by coronary artery ligation. The hearts were removed 3 hours to 2 months after MI and examined for nerve fiber density and neurotrophic factor gene expression using Affymetrix microarray and mRNA analyses. Results: The density of nerve fibers immunopositive for growth-associated protein (GAP)-43 was the highest 3 hours after MI both in the peri-infarct area and in the area remote to infarct, resulting in sympathetic (but not parasympathetic) hyperinnervation in the ventricles. The GAP-43-positive nerve fiber density of myocardium was greater in the outer transverse loop than in the inner vertical loop. The differences between these two myocardial loops peaked within 3 hours after MI and persisted for 2 months afterward. Gene expression of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α was increased up to 2 months after MI compared with normal control. Expression of these growth factors was more pronounced and persistent in the peri-infarct area than in the remote area. Conclusion: MI induces sympathetic nerve sprouting in both peri-infarct and remote areas, more in the outer transverse loop. Selective up-regulation of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α occurred in the peri-infarct area and, to a lesser extent, in the remote area.",
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T1 - Spatial distribution of nerve sprouting after myocardial infarction in mice

AU - Oh, Yong Seog

AU - Jong, Ambrose Y.

AU - Kim, Dave T.

AU - Li, Hongmei

AU - Wang, Charles

AU - Zemljic-Harpf, Alice

AU - Ross, Robert S.

AU - Fishbein, Michael C.

AU - Chen, Peng Sheng

AU - Chen, Lan S.

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Background: Myocardial infarction (MI) elicits nerve sprouting. Objectives: The purpose of this study was to determine the spatial distribution of nerve sprouting and neurotrophic gene expression after MI. Methods: We created MI in mice by coronary artery ligation. The hearts were removed 3 hours to 2 months after MI and examined for nerve fiber density and neurotrophic factor gene expression using Affymetrix microarray and mRNA analyses. Results: The density of nerve fibers immunopositive for growth-associated protein (GAP)-43 was the highest 3 hours after MI both in the peri-infarct area and in the area remote to infarct, resulting in sympathetic (but not parasympathetic) hyperinnervation in the ventricles. The GAP-43-positive nerve fiber density of myocardium was greater in the outer transverse loop than in the inner vertical loop. The differences between these two myocardial loops peaked within 3 hours after MI and persisted for 2 months afterward. Gene expression of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α was increased up to 2 months after MI compared with normal control. Expression of these growth factors was more pronounced and persistent in the peri-infarct area than in the remote area. Conclusion: MI induces sympathetic nerve sprouting in both peri-infarct and remote areas, more in the outer transverse loop. Selective up-regulation of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α occurred in the peri-infarct area and, to a lesser extent, in the remote area.

AB - Background: Myocardial infarction (MI) elicits nerve sprouting. Objectives: The purpose of this study was to determine the spatial distribution of nerve sprouting and neurotrophic gene expression after MI. Methods: We created MI in mice by coronary artery ligation. The hearts were removed 3 hours to 2 months after MI and examined for nerve fiber density and neurotrophic factor gene expression using Affymetrix microarray and mRNA analyses. Results: The density of nerve fibers immunopositive for growth-associated protein (GAP)-43 was the highest 3 hours after MI both in the peri-infarct area and in the area remote to infarct, resulting in sympathetic (but not parasympathetic) hyperinnervation in the ventricles. The GAP-43-positive nerve fiber density of myocardium was greater in the outer transverse loop than in the inner vertical loop. The differences between these two myocardial loops peaked within 3 hours after MI and persisted for 2 months afterward. Gene expression of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α was increased up to 2 months after MI compared with normal control. Expression of these growth factors was more pronounced and persistent in the peri-infarct area than in the remote area. Conclusion: MI induces sympathetic nerve sprouting in both peri-infarct and remote areas, more in the outer transverse loop. Selective up-regulation of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-β3, and interleukin-1α occurred in the peri-infarct area and, to a lesser extent, in the remote area.

KW - Cholinergic nerve

KW - DNA microarray

KW - Helical heart

KW - Myocardial infarction

KW - Nerve sprouting

KW - Neural regeneration

KW - Neural remodeling

KW - Neurotrophic factors

KW - Sympathetic nerve

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