Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells

Claire Walczak, Hailing Zong, Sachin Jain, Jane R. Stout

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The spatial and temporal control of microtubule dynamics is fundamentally important for proper spindle assembly and chromosome segregation. This is achieved, in part, by the multitude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily members, which act as microtubule-destabilizing enzymes. These fall into two general classes: the kinesin-13 proteins, which directly depolymerize microtubules, and the kinesin-8 proteins, which are plus end-directed motors that either destabilize microtubules or cap the microtubule plus ends. Here we analyze the contribution of a PtK kinesin-8 protein, Kif18B, in the control of mitotic microtubule dynamics. Knockdown of Kif18B causes defects in spindle microtubule organization and a dramatic increase in astral microtubules. Kif18Bknockdown cells had defects in chromosome alignment, but there were no defects in chromosome segregation. The long astral microtubules that occur in the absence of Kif18B are limited in length by the cell cortex. Using EB1 tracking, we show that Kif18B activity is spatially controlled, as loss of Kif18B has the most dramatic effect on the lifetimes of astral microtubules that extend toward the cell cortex. Together our studies provide new insight into how diverse kinesins contribute to spatial microtubule organization in the spindle.

Original languageEnglish (US)
Pages (from-to)3021-3030
Number of pages10
JournalMolecular Biology of the Cell
Volume27
Issue number20
DOIs
StatePublished - Oct 15 2016

Fingerprint

Microtubules
Kinesin
Chromosome Segregation
Proteins
Chromosomes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells. / Walczak, Claire; Zong, Hailing; Jain, Sachin; Stout, Jane R.

In: Molecular Biology of the Cell, Vol. 27, No. 20, 15.10.2016, p. 3021-3030.

Research output: Contribution to journalArticle

Walczak, Claire ; Zong, Hailing ; Jain, Sachin ; Stout, Jane R. / Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells. In: Molecular Biology of the Cell. 2016 ; Vol. 27, No. 20. pp. 3021-3030.
@article{b3ace75dbccc4c2f9a50c6b58ff4a037,
title = "Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells",
abstract = "The spatial and temporal control of microtubule dynamics is fundamentally important for proper spindle assembly and chromosome segregation. This is achieved, in part, by the multitude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily members, which act as microtubule-destabilizing enzymes. These fall into two general classes: the kinesin-13 proteins, which directly depolymerize microtubules, and the kinesin-8 proteins, which are plus end-directed motors that either destabilize microtubules or cap the microtubule plus ends. Here we analyze the contribution of a PtK kinesin-8 protein, Kif18B, in the control of mitotic microtubule dynamics. Knockdown of Kif18B causes defects in spindle microtubule organization and a dramatic increase in astral microtubules. Kif18Bknockdown cells had defects in chromosome alignment, but there were no defects in chromosome segregation. The long astral microtubules that occur in the absence of Kif18B are limited in length by the cell cortex. Using EB1 tracking, we show that Kif18B activity is spatially controlled, as loss of Kif18B has the most dramatic effect on the lifetimes of astral microtubules that extend toward the cell cortex. Together our studies provide new insight into how diverse kinesins contribute to spatial microtubule organization in the spindle.",
author = "Claire Walczak and Hailing Zong and Sachin Jain and Stout, {Jane R.}",
year = "2016",
month = "10",
day = "15",
doi = "10.1091/mbc.E16-04-0254",
language = "English (US)",
volume = "27",
pages = "3021--3030",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "20",

}

TY - JOUR

T1 - Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells

AU - Walczak, Claire

AU - Zong, Hailing

AU - Jain, Sachin

AU - Stout, Jane R.

PY - 2016/10/15

Y1 - 2016/10/15

N2 - The spatial and temporal control of microtubule dynamics is fundamentally important for proper spindle assembly and chromosome segregation. This is achieved, in part, by the multitude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily members, which act as microtubule-destabilizing enzymes. These fall into two general classes: the kinesin-13 proteins, which directly depolymerize microtubules, and the kinesin-8 proteins, which are plus end-directed motors that either destabilize microtubules or cap the microtubule plus ends. Here we analyze the contribution of a PtK kinesin-8 protein, Kif18B, in the control of mitotic microtubule dynamics. Knockdown of Kif18B causes defects in spindle microtubule organization and a dramatic increase in astral microtubules. Kif18Bknockdown cells had defects in chromosome alignment, but there were no defects in chromosome segregation. The long astral microtubules that occur in the absence of Kif18B are limited in length by the cell cortex. Using EB1 tracking, we show that Kif18B activity is spatially controlled, as loss of Kif18B has the most dramatic effect on the lifetimes of astral microtubules that extend toward the cell cortex. Together our studies provide new insight into how diverse kinesins contribute to spatial microtubule organization in the spindle.

AB - The spatial and temporal control of microtubule dynamics is fundamentally important for proper spindle assembly and chromosome segregation. This is achieved, in part, by the multitude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily members, which act as microtubule-destabilizing enzymes. These fall into two general classes: the kinesin-13 proteins, which directly depolymerize microtubules, and the kinesin-8 proteins, which are plus end-directed motors that either destabilize microtubules or cap the microtubule plus ends. Here we analyze the contribution of a PtK kinesin-8 protein, Kif18B, in the control of mitotic microtubule dynamics. Knockdown of Kif18B causes defects in spindle microtubule organization and a dramatic increase in astral microtubules. Kif18Bknockdown cells had defects in chromosome alignment, but there were no defects in chromosome segregation. The long astral microtubules that occur in the absence of Kif18B are limited in length by the cell cortex. Using EB1 tracking, we show that Kif18B activity is spatially controlled, as loss of Kif18B has the most dramatic effect on the lifetimes of astral microtubules that extend toward the cell cortex. Together our studies provide new insight into how diverse kinesins contribute to spatial microtubule organization in the spindle.

UR - http://www.scopus.com/inward/record.url?scp=84992150932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992150932&partnerID=8YFLogxK

U2 - 10.1091/mbc.E16-04-0254

DO - 10.1091/mbc.E16-04-0254

M3 - Article

VL - 27

SP - 3021

EP - 3030

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 20

ER -